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Article Abstract
Objective: Osteoarthritis (OA) is a chronic, debilitating disease with no available disease-modifying drugs. Biomarker identification in patients with OA has hitherto been limited to serum proteins and bulk epigenomic feature identification.
Methods: Peripheral blood mononuclear cells (PBMCs) from 21 healthy donors, 17 patients with OA, and 10 patients with degenerative meniscal tears (DMTs) were immunophenotyped at single-cell resolution by mass cytometry by time-of-flight using a 29-marker panel to identify OA-associated features in the circulating immune cells. Single-cell RNA sequencing was used to discern mechanistic attributes of perturbed immune cell populations in OA.
Results: Comparison of the PBMCs of healthy donors and OA patients revealed distinct perturbations in OA. Although subsets of naive B cells were depleted, switched memory B cells were significantly expanded in OA, including a CD25CXCR5CD27IgD subpopulation. Single-cell RNA sequencing revealed a dysfunction of interleukin 2/Stat5 and tumor necrosis factor signaling in the CD25 switched memory B cells in OA. A similar expansion of CD25 switched memory B cells was observed in patients with DMT, a population at enhanced risk for OA.
Conclusion: A CD25 switched memory B cell population was identified to be a potential cellular biomarker for OA that can be detected in the early stages of OA in the readily accessible circulating blood cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12353273 | PMC |
| http://dx.doi.org/10.1002/art.43186 | DOI Listing |
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