BAK and ARG Have Moisturizing, Anti-Inflammatory, Antioxidant, and Antioxidant Bioactivities, in the Zebrafish Model.

Background: Bakuchiol (BAK) and acetyl hexapeptide-8 have favorable biological activities and have promising applications in the cosmetic industry. However, the evaluation of the biological activity of both alone or in combination has not been reported.

Aims: The biological activity of both alone or in combination was evaluated based on the zebrafish model.

Methods: Zebrafish embryos were induced to form models using different methods. Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.

Results: BAK and ARG were able to reduce water loss from the caudal fin of zebrafish induced by 0.9% NaCl solution. They were able to alleviate the UVB-induced decrease in the expression level of the skin tightness-related gene (ELN/COL1a1b) in zebrafish embryos, and BAK or ARG effectively reversed the increase in β-galactosidase activity induced by exposure to HO solution and restored telomerase activity in zebrafish embryos. In addition, both were able to counteract oxidative stress and mitochondrial damage in zebrafish embryos as a result of LPS treatment. Finally, BAK and ARG were also effective in suppressing the increase in neutrophil counts and inflammatory cytokine levels in zebrafish embryos due to LPS exposure. Notably, BAK and ARG were more effective when used in combination.

Conclusions: Acetyl hexapeptide-8 promotes the bioactivity of BAK in zebrafish embryos (Danio rerio). BAK and ARG have moisturizing, anti-inflammatory, antioxidant, and antioxidant bioactivities in the zebrafish model.