Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Post-operative delirium (POD) is a common complication after surgery especially in elderly patients, characterized by acute disturbances in consciousness and cognition, which negatively impacts long-term outcomes. Effective treatments remain elusive due to the unclear pathophysiology of POD. To address the knowledge gap, we investigated DNA methylation profiles and gene expression changes in brain cells from POD and non-POD patients who underwent brain resection surgery for medication refractory epilepsy. DNA methylation analysis revealed alteration in epigenetic status of immune and inflammation-related genes. Single-nucleus RNA sequencing (snRNAseq) identified POD-specific glial cell alterations, particularly in microglia, where neuroinflammation was strongly enhanced, consistent with epigenetic findings. Astrocytes exhibited changes in synapse-related functions and migration. Furthermore, downstream analysis indicated similarities between POD-associated glial cell states and pathologies such as encephalitis and dementia. Overall, this study-the first multi-omics analysis of brain tissue from POD patients-provides direct evidence of glial cell contributions to POD pathogenesis, and highlights potential therapeutic targets.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952519 | PMC |
| http://dx.doi.org/10.1101/2025.03.13.643155 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prenatal alcohol exposure perturbs the development of radial glial cells in the fetal olfactory bulb.
Alcohol Clin Exp Res (Hoboken)
September 2025
Department of Neuroscience and Experimental Therapeutics, Penn State College of Medicine, Hershey, Pennsylvania, USA.
Background: Prenatal alcohol exposure (PAE) causes fetal alcohol spectrum disorder (FASD) and is associated with various cognitive and sensory impairments, including olfactory dysfunction. While both genetic and environmental factors contribute to olfactory dysfunction, PAE is considered a significant factor affecting brain development, including the olfactory system. In this study, we investigated the impact of PAE on the developing olfactory bulb (OB), specifically focusing on OB RGCs-radial glial cells that give rise to OB projection neurons.
View Article and Find Full Text PDFLaminin 332 regulates glial fibrillary acidic protein expression and astrocyte maturation during spinal cord development.
J Anat
September 2025
Department of Anatomy and Cell Biology, Hyogo Medical University School of Medicine, Nishinomiya, Hyogo, Japan.
The white matter of the spinal cord is essential for sensory and motor signaling, and its proper development is crucial for establishing functional neuronal circuits. However, the mechanisms underlying white matter formation remain incompletely understood. We hypothesized that the extracellular matrix, particularly laminins, plays a key role in this process.
View Article and Find Full Text PDFPrecise measurement of motor neuron dysfunction in Drosophila ALS model via climbing assay and leg imaging.
Methods Cell Biol
September 2025
The HIT Center for Life Sciences, Harbin Institute of Technology, Harbin, P.R. China; Medical and Health Research Institute, Zhengzhou Research Institute of HIT, Zhengzhou, HA, P.R. China. Electronic address:
Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder characterized by progressive degeneration of motor neurons, leading to muscle weakness, paralysis, and death. While there is a plethora of studies focusing on many aspects of ALS, the pathogenesis of this disease is not well understood, and effective treatments are scarce. Drosophila melanogaster is a powerful model organism for studying ALS due to its genetic tractability and its evolutionarily conserved cellular and molecular processes which are also shared between the fly and human.
View Article and Find Full Text PDFRepeatability of Myelin and Myelin Water Quantification Using Short-TR Adiabatic Inversion-Recovery Sequences.
NMR Biomed
October 2025
Department of Radiology, University of California, San Diego, California, USA.
Myelin and myelin water (MW) behavior is becoming increasingly relevant in their role in neurodegenerative diseases. Myelin proton fraction (MPF) and myelin water fraction (MWF) measured with short-TR adiabatic inversion-recovery (STAIR) sequences are potential biomarkers of myelin and MW, respectively, but their repeatabilities are unknown. This study aims to evaluate the repeatability of MPF and MWF measured with the STAIR ultrashort echo time (STAIR-UTE) and STAIR short echo time (STAIR-STE) sequences, respectively.
View Article and Find Full Text PDFEctopic recruitment of neuroblasts in striatal myelin bundles and nucleus accumbens following AraC chemical lesion.
Stem Cell Reports
September 2025
Neural Stem Cells and Neuroimaging Group, Department of Neurobiology, Hellenic Pasteur Institute, 11521 Athens, Greece. Electronic address:
In the adult brain, neural stem cells (NSCs) constitutively generate new neurons in specific neurogenic domains. Recent research has unveiled reactive neurogenesis, whereby brain injury triggers NSC activation, enhancing their differentiation potential and guiding progeny to injured areas. Our study provides evidence of alternative migration pathways for newborn neurons in the mouse subcortical forebrain, revealed by administration of a chemotherapeutic agent.
View Article and Find Full Text PDF