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Background: This case report aims to describe the diagnostic method and the clinical course of endophthalmitis caused by Enterococcus faecalis.
Case Presentation: A 66-year-old man presented with sudden, severe pain and acute vision loss in his left eye. Ocular examination revealed significant intraocular inflammation and dense vitritis, suggesting endogenous endophthalmitis. Systemic evaluation identified colonic diverticulosis as a potential source of infection. Pars plana vitrectomy was performed, and analysis of the vitreous fluid, including cytological and bacteriological studies, confirmed the presence of Enterococcus faecalis.
Conclusion: The vitreous fluid is an adequate sample for characterizing endophthalmitis by combining cytological and bacteriological analyses.
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http://dx.doi.org/10.1186/s12348-025-00482-w | DOI Listing |
Animals (Basel)
August 2025
Faculty of Public and One Health, University of Thessaly, 43100 Karditsa, Greece.
The objectives of the present work were as follows: (i) the detection of cathelicidin biomolecules in the milk of individual goats during the early stages of mastitis and their potential use for the diagnosis of mastitis at its early stage and (ii) the evaluation of the presence of cathelicidin proteins in the bulk-tank milk from goat and sheep farms. In an experimental study, after inoculation of into a mammary gland of goats, bacteriological and cytological examinations of milk samples, as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially, from 4 to 48 h post-challenge. Cathelicidin-1 and cathelicidin-2 were detected consistently in milk samples obtained throughout the study, and spot optical densities obtained from PDQuest v.
View Article and Find Full Text PDFInt J Mol Sci
June 2025
Northern Region Branch, Taiwan Livestock Research Institute, Ministry of Agriculture, Miaoli 368003, Taiwan.
Mastitis is a major issue in dairy cows, with subclinical mastitis (SCM) being hard to detect and potentially progressing to clinical mastitis. Antibiotic use raises concerns about resistance and milk contamination, highlighting the need for natural alternatives. Sodium alginate (SA), known for its antioxidant and immunomodulatory properties, may offer a solution, though its effects on mastitis are unclear.
View Article and Find Full Text PDFVet Sci
May 2025
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.
Treating endometritis is one of the major challenges faced by veterinarians. The effect of post-insemination intrauterine infusion Wharton's jelly mesenchymal stromal/stem cell-derived conditioned medium (WJ-MSC-CM) on the uterine response and pregnancy outcomes in problem mares was evaluated in this study. The cycles of 12 problem mares were treated seven/eight hours after insemination with intrauterine infusion of 20 mL of WJ-MSC-CM (TRT) or non-conditioned Ringer's lactate solution (CTR).
View Article and Find Full Text PDFAnim Reprod Sci
June 2025
Center of Reproductive Physiology and Diagnostic Imaging, Faculty of Veterinary Sciences of the National University of La Plata, Argentina; CONICET, Argentina.
The aims of this study were: 1) To evaluate the diagnostic value of endometrial cytological samples obtained by endoscopically guided transcervical catheterization (TCC) in canine endometritis and normal uterus; 2) To determine the sensitivity and specificity of TCC for the identification of uterine bacterial growth; 3) To compare vaginal and uterine bacteriological findings. Before elected ovariohysterectomy, vaginal and TCC bacteriological and cytological samples were obtained in ten post-pubertal bitches. After surgery, direct uterine bacteriological sampling and full-thickness biopsy were also carried out.
View Article and Find Full Text PDFSci Rep
May 2025
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
This study was conducted aiming to investigate impacts of experimentally induced endometritis on the vascular perfusion and echogenicity of the endometrium in dairy cows. Following estrus synchronization and applying cytological and bacteriological examinations, Holstein cows (n = 9) were enrolled in the experiment. The endometrial blood flow and echogenicity in the middle of each uterine horn were evaluated two times a week until the subsequent estrus by power Doppler and two dimensional ultrasonography.
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