Exploiting TRP channel diversity in insects: a pathway to next-generation pest management.

Transient Receptor Potential (TRP) channels, a diverse family of over 30 ion channel subtypes, are pivotal in regulating sensory perception, thermoregulation, and feeding in insects. In Drosophila melanogaster, 13 TRP channels have been identified, while Aedes aegypti and Anopheles gambiae possess 11 and 10, respectively, showcasing evolutionary adaptations to their ecological niches. This review explores recent advancements in understanding the structure, classification, and physiological functions of TRP channels, emphasizing their evolutionary divergence across Diptera, Lepidoptera, and Hymenoptera. Key TRP subfamilies, such as TRPA, TRPC, TRPM, TRPV, TRPN, and TRPP, are discussed, highlighting their roles in chemo-sensation, gustation, and stress responses. Examples include TRPA1's involvement in thermal sensing and TRPV's role in osmoregulation, critical for insect survival under fluctuating environmental conditions. The review highlights the potential of TRP channels as targets for pest control, focusing on TRP-specific insecticides like pymetrozine, afidopyrifen, and flonicamid, which impact feeding and sensory pathways. RNA interference (RNAi) techniques targeting TRP genes are highlighted as promising tools for innovative pest management. TRP channels' role in mediating thermal tolerance is particularly significant in the context of climate change, where variable temperatures challenge pest dynamics and agricultural sustainability. Understanding these mechanisms is vital for developing climate-resilient pest control strategies. The review also evaluates methodologies used in TRP channel studies, including genomic, transcriptomic, and functional assays, alongside behavioural analyses. Despite progress, challenges remain in studying TRP channels in non-model insects and elucidating their regulation. Future research should integrate multidisciplinary approaches to fully harness TRP channels for sustainable pest management.