Multi-Shank 1024 Channels Active SiNAPS Probe for Large Multi-Regional Topographical Electrophysiological Mapping of Neural Dynamics.

Implantable active dense CMOS neural probes unlock the possibility of spatiotemporally resolving the activity of hundreds of single neurons in multiple brain circuits to investigate brain dynamics. Mapping neural dynamics in brain circuits with anatomical structures spanning several millimeters, however, remains challenging. Here, a CMOS neural probe advancing lateral sampling for mapping intracortical neural dynamics (both LFPs and spikes) in awake, behaving mice from an area >4 mm is demonstrated. By taking advantage of SiNAPS technology modularity, an 8-shank probe with 1024 recording channels arranged in regular arrays of 128 electrodes/shank with an electrode pitch <30 µm is realized. Continuous low-noise recordings (spikes with 6.67 ± 1.02 µV) from all 1024 electrodes at 20 kHz/channel demonstrate the monitoring at high spatial and temporal resolution of a field of view spanning the 2D lattice of the entire mice hippocampal circuit, together with cortical and thalamic regions. This arrangement allows combining large population unit monitoring across distributed networks with precise intra- and interlaminar/nuclear mapping of the oscillatory dynamics.