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Brazil is a global leader in the orange industry, producing approximately one-fourth of the world's oranges and generating over 50% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach-microwave-assisted extraction-to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66%) and HPLC (84.30%) analyses.
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http://dx.doi.org/10.3390/foods14040642 | DOI Listing |
Talanta
September 2025
Department of Physical and Environmental Sciences, University of Toronto, Scarborough, Toronto, Ontario, M1C 1A4, Canada. Electronic address:
An one-pot method was used to prepare bimetallic nanozymes, with chitosan (CS) and l-tyrosine (L-Tyr) as stabilized dispersed colloidal solutions and a carrier for gold-platinum single atoms (Au-Pt SAs), which exhibited excellent peroxidase activity. A colorimetric method based on CS/L-Tyr/Au-Pt SAs nanozymes was constructed for the colorimetric detection of quercetin (QR) in human serum and orange juice. The synthesized bimetallic nanozymes were characterized by SEM, TEM, HAADF-STEM, FT-IR, XRD and XPS techniques to demonstrate the successful synthesis of CS/L-Tyr/Au-Pt SAs nanozymes.
View Article and Find Full Text PDFJ Vis
August 2025
Western Kentucky University, Bowling Green, KY, USA.
The physical interactions among objects in the natural environment can cause dramatic changes in their shapes or patterns of motion, and those changes can provide reliable information to distinguish different types of events or materials. The present research was designed to investigate the identification of fluid materials. Observers viewed computer animations and static images of a shiny orange translucent fluid flowing from a tube into a glass jar, and they were asked to make confidence ratings about whether the depicted material looked like water/juice, oil/paint, honey/molasses, or caulk/toothpaste.
View Article and Find Full Text PDFBiol Trace Elem Res
August 2025
Laboratory of Materials, Faculty of Sciences, Mohammed V University in Rabat, Avenue Ibn Battouta, BP:1014, 10000, Nanotechnologies, and Environment,Rabat , Morocco.
This study aimed to determine the levels of lead (Pb) and cadmium (Cd) in orange juice samples collected from various markets in Rabat (Morocco), using graphite furnace atomic absorption spectrometry (GF-AAS), and to evaluate the risk posed to consumer health. Pb was detected in 58% of samples, with concentrations ranging from 0.009 to 0.
View Article and Find Full Text PDFMikrochim Acta
August 2025
State Key Laboratory of Pathogen and Biosecurity, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) is an important zoonotic pathogen threatening global food safety. The development of rapid and sensitive on-site detection technologies is of great significance for preventing foodborne disease outbreaks. In this study, a tri-mode portable biosensor integrating magnetic separation (FeO), nanozyme catalysis (ZIF-8@GOx@PtNPs), and smartphone assisted detection was constructed for the point-of-care detection of EHEC O157:H7.
View Article and Find Full Text PDFMikrochim Acta
August 2025
Laboratory of Sensors, Nanomedicine, and Nanostructured Materials, Federal University of São Carlos, Araras, São Paulo, 13604-900, Brazil.
A laser-pyrolyzed cork-based electrochemical sensor is proposed for the determination of sodium nitrite, a widely used food additive that poses potential health risks. The pyrolysis parameters were varied individually, and electrochemical performance was compared to select the conditions that provided the highest conductivity and sensitivity. Additionally, a chemical treatment with a waterproofing spray was applied to enhance sensor stability and ensure reproducible measurements.
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