Application Progress of Immobilized Enzymes in the Catalytic Synthesis of 1,3-Dioleoyl-2-palmitoyltriglyceride Structured Lipids.

In recent years, the preparation of OPO (1,3-dioleoyl-2-palmitoyltriglyceride)-structured lipids through immobilized lipase catalysis has emerged as a research hotspot in the fields of food and biomedical sciences. OPO structured lipids, renowned for their unique molecular structure and biological functions, find wide applications in infant formula milk powder, functional foods, and nutritional supplements. Lipase-catalyzed reactions, known for their efficiency, high selectivity, and mild conditions, are ideal for the synthesis of OPO structured lipids. Immobilized lipases not only address the issues of poor stability and difficult recovery of free enzymes but also enhance catalytic efficiency and reaction controllability. This review summarizes the latest advancements in the synthesis of OPO structured lipids using immobilized lipases, focusing on immobilization methods, enhancements in enzyme activity and stability, the optimization of reaction conditions, and improvements in product purity and yield. Furthermore, it delves into the reaction mechanisms of enzymatic synthesis of OPO structured lipids, process optimization strategies, and the challenges and broad prospects faced during industrial applications.