Green and Efficient Extraction of Natural Rubber and Its Structural Analysis.

Natural rubber (NR) is in high demand due to its excellent elasticity and physical and mechanical properties, but production is limited and NR is in short supply. There is an urgent need to find new alternative rubber sources. (TKS), as a green, renewable, widely planted and high content rubber producing plant, has shown broad application prospects. The extraction process is the key to developing efficient, green, and high-purity Natural Rubber (TKNR) to replace NR in various applications. In this study, TKS roots were processed through repeated boiling to remove inulin, followed by alkaline treatment with potassium hydroxide (KOH) to isolate lignin and facilitate cell wall disruption. Subsequent enzymatic hydrolysis using pectinase and cellulase enabled the dissolution of root-structure carbohydrates, thereby obtained TKNR. Structural characterization of TKNR was conducted and compared with that of NR. The results showed that the combined alkaline and enzymatic extraction methodology effectively isolates TKNR from TKS roots. Structural analysis reveals that TKNR closely resembles NR, having comparable molecular weight and distribution, crystallinity, and crosslinking networks, with both polymers primarily consisting of cis-1,4-polyisoprene.