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Article Abstract
Background: Our previous research revealed that daphnetin (7,8-dihydroxycou-marin) positively influences the balance between forked transcription factor P3 (+) regulatory T cells (Treg) and T helper 17 (Th17) cells in the peripheral blood mononuclear cells of individuals with unexplained recurrent pregnancy loss. However, the specific mechanism remains unclear. This research aims to further examine how daphnetin regulates the Th17 cell/+ Treg cell imbalance in a mouse model with unexplained recurrent spontaneous abortion (URSA).
Methods: Mice (n = 40) were allocated into the following groups: daphnetin high dose (4 mg/kg·day), daphnetin low dose (1 mg/kg·day), URSA model, and normal pregnancy (control). We used flow cytometry for assessing the Th17/Treg cell ratio in peripheral blood mononuclear cells, quantitative real-time polymerase chain reaction for measuring cytokine expression levels, and transmission electron microscopy for observing ultrastructural changes in decidual tissues and calculating the embryo absorption rate.
Results: Compared to the URSA model group, daphnetin significantly reduced the T17cell/+ Treg cell ratio in peripheral blood mononuclear cells. Daphnetin also decreased the expression of Th17 cell-related cytokines, including orphan nuclear receptor γt () and signal transduction and transcriptional activator 3 (STAT3), as well as increase the expression of + Treg cells-related cytokines, including STAT5 and +. Furthermore, daphnetin reduced the embryo absorption rate and improved the decidual tissue ultrastructure of URSA model mice.
Conclusion: Daphnetin improves the Th17 cell/+ Treg cell imbalance in URSA model mice, thereby contributing to the repair of decidual tissue damage and reducing the embryo absorption rate. These findings suggest that daphnetin may offer a new method for treating URSA.
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| http://dx.doi.org/10.24976/Discov.Med.202436190.199 | DOI Listing |
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Article Synopsis
- Daphnetin shows potential in correcting the imbalance between Th17 cells and Treg cells in a mouse model of unexplained recurrent spontaneous abortion (URSA), as indicated by decreased ratios of these cells.
- The research involved various dosing groups of mice, with numerous methods used to analyze changes in cell ratios, cytokine expression, and tissue structure.
- Results show that daphnetin not only reduces harmful Th17 cell-related cytokines but also enhances beneficial Treg cell-related cytokines, leading to lower embryo absorption rates and improved tissue structure.