A multiple biomolecules-based rapid life detection protocol embedded in a rover scientific subsystem for soil sample analysis.

The study of whether life exists, is extinct, or not depends on various sophisticated experimental studies, as many different signatures of life can be used. The experimental procedures that can be performed to identify life can be further restricted by time, resources, and mobility constraints. Therefore, any research analyzing the presence of extraterrestrial life must be precise and unambiguous. This research focuses on the objective of the extraterrestrial life detection domain and seeks to provide an efficient protocol that can produce life detection decisions based on empirical data obtained through chemical analysis under time and resource-constrained conditions. While the majority of existing frameworks in this field are designed to identify biomolecules, our goal is to accomplish the same with minimal operational expense and mission complexity. We argue that the thoughtful integration of multiple biomolecular detections with lesser complexity and a robust framework can improve overall mission performance by satisfying the necessary time and resource constraints. In this study, a rapid multiple biomolecules-based life detection protocol (MBLDP-R) from soil samples is developed from scratch and embedded in an operational scientific rover subsystem targeted for planetary analysis missions. The study uses artificial biomolecule samples and simulated extraterrestrial environments to illustrate the suggested protocol's end-to-end process. First, we list a few significant biomolecules, including lipids, proteins, carbohydrates, nucleic acids, ammonia, and pigments. Then, a weighted qualitative test scoring is applied to sort out the best test method for the finally selected biomolecules which are used as operational analogue to showcase the protocol's in-situ analysis and decision-making capabilities. Based on the suitable biomolecules, a scientific exploration subsystem is developed, and the implemented protocol is built to perform onboard sample analysis. Evaluation results show that: (1) the proposed MBLDP-R protocol could effectively predict the classes with an average f1-score of 98.65% (macro) and 90.00% (micro), (2) the area under the Receiver Operating Characteristics (AUC-ROC) curve shows the sample categories to be correctly predicted 92% of the time (97% for Extant, 88% for Extinct, and 92% in the case of NPL), and (3) the protocol is time-efficient with an average completion time of 17.60 min, demonstrating the protocol's rapid nature in detecting biosignatures in soil samples. The research outcome yields useful additional data for related future studies, particularly in the design of scientific frameworks for mission-specific requirements with limited resources while also serving as a reference point for constraint evaluation methods for similar systems.