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DNA molecules, as a storage medium, possess unique advantages. Not only does DNA storage exhibit significantly higher storage density compared to electromagnetic storage media, but it also features low energy consumption and extremely long storage times. However, the integration of DNA storage into daily life remains distant due to challenges such as low storage density, high latency, and inevitable errors during the storage process. Therefore, this paper proposes constructing a DNA storage coding set based on the Levy Sooty Tern Optimization Algorithm (LSTOA) to achieve an efficient random-access DNA storage system. Firstly, addressing the slow iteration speed and susceptibility to local optima of the Sooty Tern Optimization Algorithm (STOA), this paper introduces Levy flight operations and propose the LSTOA. Secondly, utilizing the LSTOA, this paper constructs a DNA storage encoding set to facilitate random access while meeting combinatorial constraints. To demonstrate the coding performance of the LSTOA, this paper consists of analyses on 13 benchmark test functions, showcasing its superior performance. Furthermore, under the same combinatorial constraints, the LSTOA constructs larger DNA storage coding sets, effectively reducing the read-write latency and error rate of DNA storage.
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http://dx.doi.org/10.3390/e26090778 | DOI Listing |
ACS Synth Biol
September 2025
Department of BioSciences, Rice University, MS-140, 6100 Main Street, Houston, Texas 77005, United States.
Microbes can be programmed to record participation in gene transfer by coding biological-recording devices into mobile DNA. Upon DNA uptake, these devices transcribe a catalytic RNA (cat-RNA) that binds to conserved sequences within ribosomal RNAs (rRNAs) and perform a trans-splicing reaction that adds a barcode to the rRNAs. Existing cat-RNA designs were generated to be broad-host range, providing no control over the organisms that were barcoded.
View Article and Find Full Text PDFJ Anim Sci
September 2025
USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, 68933 USA.
Low-coverage sequencing refers to sequencing DNA of individuals to a low depth of coverage (e.g., 0.
View Article and Find Full Text PDFFood Res Int
November 2025
Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil. Electronic address:
The consumption of water of low microbiological quality can be detrimental and may cause significant health issues. Thus, amplicon sequencing can be an advantageous method to observe bacterial diversity in water. This study aimed to understand the complex bacterial communities present in natural mineral water packaged in 20 L returnable containers through amplicon sequencing.
View Article and Find Full Text PDFNeuropsychopharmacol Rep
September 2025
Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
Controlling for confounding factors in postmortem brain studies of psychiatric disorders is crucial, particularly in gene expression analyses. Potential confounding factors include sex, age at death, medication history, agonal state, postmortem interval (PMI), tissue storage duration, tissue pH, and RNA integrity number (RIN). pH and RIN are considered particularly important in gene expression analysis because they accurately reflect mRNA quality.
View Article and Find Full Text PDFJ Pharm Sci
September 2025
School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address:
Ferritin is a shell-like carrier protein with an 8 nm diameter cavity that naturally provides a space for encapsulating food and drug components. In the absence of iron atoms bound to this protein, it is called apoferritin, the form used in this study. However, its vulnerability to environmental conditions when used alone warrants further investigation.
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