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Article Abstract
Background: is a significant malaria vector in Pakistan, and understanding its feeding behavior is necessary to control the spread of malaria. However, limited information is available on the host preferences of in Pakistan. Therefore, we aimed to explore the feeding behavior of , a malaria vector, in the District Khyber, Khyber Pakhtunkhwa, Pakistan.
Methods: A total of 7462 mosquitoes were collected between March and September 2021, with 1674 (22.4%) identified as (952 female and 722 male). Among the female , 495 (52%) were blood-fed. DNA was extracted from the blood-fed female mosquitoes using the Ammonium Acetate Precipitation Method followed by PCR analysis, blood meal sources were identified. Nested PCR on 191 pooled samples was used to detect and .
Results: Cattle blood meals were predominant (73%), followed by human (20%) and chicken (7%), with no dog blood meals detected. All individual mosquito samples were negative for , while two pooled samples (out of 191) tested positive for .
Conclusion: in Khyber District primarily displayed anthropophagic feeding behavior, with a small portion of the population infected with . The results underscore the importance of targeted vector control strategies, environmental management, community engagement and continuous monitoring to suppress malaria transmission.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417983 | PMC |
| http://dx.doi.org/10.18502/ijpa.v19i3.16393 | DOI Listing |
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