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Unsupervised domain adaptation medical image segmentation is aimed to segment unlabeled target domain images with labeled source domain images. However, different medical imaging modalities lead to large domain shift between their images, in which well-trained models from one imaging modality often fail to segment images from anothor imaging modality. In this paper, to mitigate domain shift between source domain and target domain, a style consistency unsupervised domain adaptation image segmentation method is proposed. First, a local phase-enhanced style fusion method is designed to mitigate domain shift and produce locally enhanced organs of interest. Second, a phase consistency discriminator is constructed to distinguish the phase consistency of domain-invariant features between source domain and target domain, so as to enhance the disentanglement of the domain-invariant and style encoders and removal of domain-specific features from the domain-invariant encoder. Third, a style consistency estimation method is proposed to obtain inconsistency maps from intermediate synthesized target domain images with different styles to measure the difficult regions, mitigate domain shift between synthesized target domain images and real target domain images, and improve the integrity of interested organs. Fourth, style consistency entropy is defined for target domain images to further improve the integrity of the interested organ by the concentration on the inconsistent regions. Comprehensive experiments have been performed with an in-house dataset and a publicly available dataset. The experimental results have demonstrated the superiority of our framework over state-of-the-art methods.
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http://dx.doi.org/10.1109/TIP.2024.3451934 | DOI Listing |
Physiology (Bethesda)
September 2025
Departments of Ophthalmology and Medicine, Stanford Cardiovascular Institute, Stanford University, Palo Alto, CA 94304.
Canonical activation of G-protein coupled receptors (GPCRs) by hormone binding occurs at the plasma membrane, resulting in the diffusion of second messengers to intracellular effector sites throughout the cell. In contrast, recent evidence suggests that functional GPCRs can induce signaling from distinct intracellular domains, contributing to specificity in signaling. Functional adrenergic receptors have been identified at intracellular sites in the cardiac myocyte such as endosomes, the sarcoplasmic reticulum, the Golgi and the inner nuclear membrane.
View Article and Find Full Text PDFBioinformatics
September 2025
Computational Health Center, Helmholtz Center Munich, Neuherberg, 85764, Germany.
Motivation: Recent pandemics have revealed significant gaps in our understanding of viral pathogenesis, exposing an urgent need for methods to identify and prioritize key host proteins (host factors) as potential targets for antiviral treatments. De novo generation of experimental datasets is limited by their heterogeneity, and for looming future pandemics, may not be feasible due to limitations of experimental approaches.
Results: Here we present TransFactor, a computational framework for predicting and prioritizing candidate host factors using only protein sequence data.
Bioinformatics
September 2025
Biocomputation and Complex Systems Physics Institute (BIFI)-Joint Unit GBsC-CSIC, University of Zaragoza, Zaragoza, 50018, Spain.
Motivation: The stability of protein interfaces influences protein dynamics and unfolding cooperativity. Although in some cases the dynamics of proteins can be deduced from their topology, much of the stability of an interface is related to the complementarity of the interacting parts. It is also important to note that proteins that display non-cooperative unfolding cannot be rationally stabilized unless the regions that unfold first are known.
View Article and Find Full Text PDFEmerg Microbes Infect
December 2025
School of Global Health, Chinese Centre for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
There is no vaccine for severe malaria. STEVOR antigens on the surface of -infected red blood cells are implicated in severe malaria and are targeted by neutralizing antibodies, but their epitopes remain unknown. Using computational immunology, we identified highly immunogenic overlapping B- and T-cell epitopes (referred to as multiepitopes, 7-27 amino acids) in the semiconserved domain of four STEVORs linked with severe malaria and clinical immunity.
View Article and Find Full Text PDFIEEE J Biomed Health Inform
September 2025
The tumor microenvironment is a dynamic eco system where cellular interactions drive cancer progression. However, inferring cell-cell communication from non-spatial scRNA-seq data remains challenging due to incomplete li gand-receptor databases and noisy cell type annotations. H ere, we propose scGraphDap, a graph neural network frame work that integrates functional state pseudo-labels and graph structure learning to improve both cell type annotation an d CCC inference.
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