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http://dx.doi.org/10.1002/mdc3.14176 | DOI Listing |
Plant Physiol
September 2025
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
In this study, we used stable isotope labeling coupled with reversed-phase HPLC-MS to annotate the origin of metabolite features in Arabidopsis (Arabidopsis thaliana) (Columbia-0) seedling rosettes and stems. Using this strategy, a total of 1,240 metabolite features were shown to be derived from 15 amino acids, and these represented 10% to 30% of the total ion counts detected by untargeted LC-MS. The amino acid-derived metabolomes (AADMs) of rosettes and stems exhibited differing patterns of accumulation.
View Article and Find Full Text PDFbioRxiv
August 2025
Department of Biochemistry and Molecular Biology, Michigan State University, MI, USA.
The nickel pincer nucleotide (NPN) cofactor catalyzes the racemization/epimerization of α-hydroxy acids in enzymes of the LarA family. The established proton-coupled hydride transfer mechanism requires two catalytic histidine residues that alternately act as general acids and general bases. Notably, however, a fraction of LarA homologs (LarAHs) lack one of the active site histidine residues, replacing it with an asparaginyl side chain that cannot participate in acid/base catalysis.
View Article and Find Full Text PDFbioRxiv
August 2025
Microbiology, School of Biological and Chemical Sciences, University of Galway, Ireland.
Alanine transport and metabolism impact MRSA pathophysiology by dictating the availability of d-alanine for cell wall synthesis, the target of β-lactam antibiotics. Furthermore -dependent alanine transport controls MRSA β-lactam susceptibility in chemically defined medium (CDM) in a glucose-dependent manner. Here we report that was auxotrophic for l-alanine in CDM, and that this growth defect was rescued by glucose (or compensatory mutations), but only when the alanine racemase () and d-alanine aminotransferase () genes were functional.
View Article and Find Full Text PDFGenomics
August 2025
College of Biology and Oceanography, Weifang University, Weifang 261061, China.
Bioluminescence in fireflies is dependent on luciferin metabolism in luminous organs. Our study applied RNA-seq to compare the transcriptome profiles between the luminous and non-luminous tissues from the larvae of the firely Pyrocoelia pectoralis. Genes that were differentially expressed between the two tissue samples were screened to identify candidate genes involved in luciferin metabolism.
View Article and Find Full Text PDFJ Inherit Metab Dis
September 2025
Charles Dent Metabolic Unit, National Hospital for Neurology and Neurosurgery, London, UK.
Many inborn errors of metabolism affect pathways involved in the synthesis of a metabolite that has an important biochemical or physiological function, and adverse effects of the disorder can be attributed to the lack of this metabolite. Thus, there is the opportunity for treatment by 'product replacement'. One of the disorders in the pathways for the synthesis of bile acids from cholesterol, 3β-hydroxy-Δ5-C27-steroid dehydrogenase deficiency, causes cholestatic liver disease in infancy that can be treated very effectively with chenodeoxycholic acid (CDCA) and/or cholic acid (CA).
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