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Article Abstract
Purpose: To investigate the genetic diversity of IncG plasmids, we have proposed a typing scheme based on replicon and performed comparative genomic analysis of five IncG plasmids from China.
Methods: p30860-KPC, p116965-KPC, pA1705-KPC, pA1706-KPC and pNY5520-KPC total in five IncG plasmids from clinical isolates of and , respectively, were fully sequenced and were compared with the previously collected reference plasmid p10265-KPC.
Results: Based on phylogeny, IncG-type plasmids are divided into IncG-I to IncG-VIII, the five plasmids belong to IncG-VIII. A detailed sequence comparison was then presented that the IncG plasmid involved accessory region I (Tn), accessory region II (IS), and accessory region III ( -region). Expect for the pNY5520-KPC, the rest of the plasmids had the same backbone structure as the reference one. Within the plasmids, insertion sequences Tn and Tn were identified, a novel unknown insertion region was found in Tn. In addition, Tn and Tn were newly designated in the study.
Conclusion: The data presented here including a typing scheme and detailed genetic comparison which provide an insight into the diversification and evolution history of IncG plasmids.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11265224 | PMC |
| http://dx.doi.org/10.2147/IDR.S461039 | DOI Listing |
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