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Recent advancements in plant regeneration and synthetic polyploid creation have been documented in Gossypium arboreum ZB-1. These developments make ZB-1 a potential model within the Gossypium genus for investigating gene function and polyploidy. This work generated the sequence and annotation of the ZB-1 genome. The contig-level genome was constructed using the PacBio high-fidelity reads, encompassing 81 contigs with an N50 length of 112.12 Mb. The Hi-C data assisted the construction of the chromosome-level genome, which consists of 13 pseudo-chromosomes and 39 un-anchored contigs, with a total length of about 1.67 Gb. Repetitive sequences accounted for about 69.7% of the genome in length. Based on ab initio and evidence-based prediction, we have identified 48,021 protein-coding genes in the ZB-1 genome. Comparative genomics analysis revealed conserved gene content and arrangement between ZB-1 and G. arboreum SXY1. The single nucleotide polymorphism occurrence rate between ZB-1 and SXY1 was about 0.54 per 1,000 nucleotides. This study enriched the genomic resources for further exploration into cotton regeneration and polyploidy mechanisms.
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http://dx.doi.org/10.1038/s41597-024-03481-z | DOI Listing |
Int J Syst Evol Microbiol
September 2025
School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the and genera of the order , but they did not exactly match any known species.
View Article and Find Full Text PDFJ Hered
September 2025
Institute of Fishery Science, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China.
Nuclear mitochondrial DNA segments (NUMTs), which are mitochondrial DNA fragments integrated into the nuclear genome, serve as markers of evolutionary history. This study aims to enhance the detection and analysis of NUMTs by developing a script named NUMTsearcher. Utilizing the latest chromosome-level genome assemblies from various species, including human, rabbit, and six fish species, the study compares NUMTsearcher's performance against traditional methods such as LAST (Local Alignment Search Tool), BLAST (Basic Local Alignment Search Tool), BLAT (BLAST-Like Alignment Tool), and the pan-mitogenome approach, which integrates mitogenomes from diverse sources to identify fixed NUMTs in the nuclear genome.
View Article and Find Full Text PDFIMA Fungus
August 2025
State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China Institute of Microbiology, Chinese Academy of Sciences Beijing China.
is a widely consumed edible mushroom and the only species currently cultivated on an industrial scale. Despite its economic importance, its trophic strategy and genomic adaptations remain elusive. Here, we presented high-quality, chromosome-level genome assemblies for two sexually compatible monokaryons (PP78 and PP85) of .
View Article and Find Full Text PDFNew Phytol
September 2025
State Key Laboratory of Plant Diversity and Specialty Crops/Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
Heterostyly is a polymorphic floral adaptation controlled by supergenes. The molecular basis of distyly has been investigated in diploid species from several unrelated families, but information is lacking for polyploid systems. Here, we address this knowledge gap in Schizomussaenda henryi, a tetraploid distylous species of Rubiaceae, the family with the greatest number of heterostylous species.
View Article and Find Full Text PDFBiology (Basel)
July 2025
Characteristic Laboratory of Forensic Science in the Universities of Shandong Province, Shandong University of Political Science and Law, Jinan 250014, China.
, as one of the common blowflies, displays biological characteristics, such as ovoviviparity and carrion-feeding adaptation. Thus, this species is generally considered of significant ecological, medical, and forensic importance. However, without a high-quality pseudo-chromosome genome for , elucidating its evolutionary trajectory proved difficult.
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