A Guide to Biodetection in Droplets.

Anal Chem

Department of Chemistry and Biotechnology, Tallinn University of Technology (TalTech), Akadeemia tee 15, Tallinn 12618, Estonia.

Published: June 2024


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Droplet-based methods for optical biodetection enable unprecedented high-throughput experimental parameters. The methods, however, remain underused due to the accompanying multidisciplinary and complicated experimental workflows. Here, we provide a tutorial for droplet-based optical biodetection workflows with a focus on the key aspect of label selection. By discussing and guiding readers through recent state-of-the-art studies, we aim to make droplet-based approaches more accessible to the general scientific public.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11190884PMC
http://dx.doi.org/10.1021/acs.analchem.3c04282DOI Listing

Publication Analysis

Top Keywords

optical biodetection
8
guide biodetection
4
biodetection droplets
4
droplets droplet-based
4
droplet-based methods
4
methods optical
4
biodetection enable
4
enable unprecedented
4
unprecedented high-throughput
4
high-throughput experimental
4

Similar Publications

Water-insensitive down-shifting nanoparticles for sensitive biosensing.

Light Sci Appl

September 2025

Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai, 200433, China.

Conventional optical probes suffer from signal degradation in aqueous media, hindering sensitive biodetection. To overcome this, newly developed water-insensitive down-shifting nanoparticles (WINPs) possess superior photophysical properties in the NIR-I window, including high quantum yield and negligible thermal effects, permitting stable, high-contrast signal generation under low excitation power. This advantage facilitated a low-power lateral flow assay capable of highly sensitive avian influenza virus (AIV) detection in the opaque biological matrices (such as avian swabs), mitigating interference issues relying on visible-range signals.

View Article and Find Full Text PDF

Mixed metal metal-organic framework synergized with functional liposomes boosts bipolar-driven organic photoelectrochemical transistor for highly sensitive biodetection.

Biosens Bioelectron

August 2025

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:

Organic photoelectrochemical transistor (OPECT) has emerged as a promising platform for investigating photoactive biomolecular interactions and advancing bioanalytical detection systems. However, many important challenges and hurdles remain implementing high gating effects and sensitive biosensing detection caused by the inherent limitations of the configuration of the photoelectrode structures and innovative biosensing. Inspired by the self-powered photoelectrochemical (PEC) systems and liposomes-assisted bioanalysis for signal amplification, a bipolar-driven poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS) OPECT based on CdS/Mixed Metal Metal-Organic Framework (MM-MOF) photoanode and a poly(1,4-diethynylbenzene) (pDEB) cathode is proposed, and exhibits a considerable current gain of ca.

View Article and Find Full Text PDF

The precise modulation of circular dichroism (CD) is essential for applications in polarization control, sensing, and imaging. Here, we report a novel, to the best of our knowledge, dual-CD response mechanism based on bound states in the continuum (BICs) in an all-dielectric silicon metasurface. By leveraging two distinct topologically protected BICs and converting them into circularly polarized eigenstates, we first demonstrate a reversal of maximum chirality at oblique incidence without requiring any structural alteration.

View Article and Find Full Text PDF

ConspectusInorganic nanomaterials typically exhibit a wide variety of structures with flexibility and versatile functional properties. The introduction of chirality can influence the physicochemical properties of materials, such as their size, shape, crystal structure, surface charge and optical activity. These properties can directly affect the fate of chiral inorganic nanomaterials.

View Article and Find Full Text PDF

Herein, we report the development of a novel sensor combining electrochemistry, localized surface plasmon resonance (LSPR) in nanoparticles, and fiber-optic sensing for the first time. The sensor developed comprises an indium tin oxide (ITO)-coated multimodal optical fiber with gold nanoparticles immobilized on the ITO surface via electrostatic self-assembly. Two types of sensing methods are demonstrated: one based on shift in LSPR peak wavelength accompanying electrochemical oxidation-reduction of an analyte and the other based on potential scanning-based detection of refractive index change.

View Article and Find Full Text PDF