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Droplet-based methods for optical biodetection enable unprecedented high-throughput experimental parameters. The methods, however, remain underused due to the accompanying multidisciplinary and complicated experimental workflows. Here, we provide a tutorial for droplet-based optical biodetection workflows with a focus on the key aspect of label selection. By discussing and guiding readers through recent state-of-the-art studies, we aim to make droplet-based approaches more accessible to the general scientific public.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11190884 | PMC |
http://dx.doi.org/10.1021/acs.analchem.3c04282 | DOI Listing |
Light Sci Appl
September 2025
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai, 200433, China.
Conventional optical probes suffer from signal degradation in aqueous media, hindering sensitive biodetection. To overcome this, newly developed water-insensitive down-shifting nanoparticles (WINPs) possess superior photophysical properties in the NIR-I window, including high quantum yield and negligible thermal effects, permitting stable, high-contrast signal generation under low excitation power. This advantage facilitated a low-power lateral flow assay capable of highly sensitive avian influenza virus (AIV) detection in the opaque biological matrices (such as avian swabs), mitigating interference issues relying on visible-range signals.
View Article and Find Full Text PDFBiosens Bioelectron
August 2025
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:
Organic photoelectrochemical transistor (OPECT) has emerged as a promising platform for investigating photoactive biomolecular interactions and advancing bioanalytical detection systems. However, many important challenges and hurdles remain implementing high gating effects and sensitive biosensing detection caused by the inherent limitations of the configuration of the photoelectrode structures and innovative biosensing. Inspired by the self-powered photoelectrochemical (PEC) systems and liposomes-assisted bioanalysis for signal amplification, a bipolar-driven poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS) OPECT based on CdS/Mixed Metal Metal-Organic Framework (MM-MOF) photoanode and a poly(1,4-diethynylbenzene) (pDEB) cathode is proposed, and exhibits a considerable current gain of ca.
View Article and Find Full Text PDFThe precise modulation of circular dichroism (CD) is essential for applications in polarization control, sensing, and imaging. Here, we report a novel, to the best of our knowledge, dual-CD response mechanism based on bound states in the continuum (BICs) in an all-dielectric silicon metasurface. By leveraging two distinct topologically protected BICs and converting them into circularly polarized eigenstates, we first demonstrate a reversal of maximum chirality at oblique incidence without requiring any structural alteration.
View Article and Find Full Text PDFAcc Chem Res
August 2025
Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
ConspectusInorganic nanomaterials typically exhibit a wide variety of structures with flexibility and versatile functional properties. The introduction of chirality can influence the physicochemical properties of materials, such as their size, shape, crystal structure, surface charge and optical activity. These properties can directly affect the fate of chiral inorganic nanomaterials.
View Article and Find Full Text PDFAnal Chem
June 2025
Department of Natural and Environmental Sciences, Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
Herein, we report the development of a novel sensor combining electrochemistry, localized surface plasmon resonance (LSPR) in nanoparticles, and fiber-optic sensing for the first time. The sensor developed comprises an indium tin oxide (ITO)-coated multimodal optical fiber with gold nanoparticles immobilized on the ITO surface via electrostatic self-assembly. Two types of sensing methods are demonstrated: one based on shift in LSPR peak wavelength accompanying electrochemical oxidation-reduction of an analyte and the other based on potential scanning-based detection of refractive index change.
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