Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Enteroids are in vitro models to study gastrointestinal pathologies and test personalized therapeutics; however, the inherent complexity of enteroids often renders standard gene editing approaches ineffective. Here, we introduce a refined lentiviral transfection protocol, ensuring sufficient lentiviral engagement with enteroids while considering spatiotemporal growth variability throughout the extracellular matrix. Additionally, we highlight a selection process for transduced cells, introduce a protocol to accurately measure transduction efficiency, and explore methodologies to gauge effects of gene knockdown on biological processes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11145376 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103082 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Smartphone-assisted image analysis for orthophosphate in aqueous samples with CTAB-enhanced extraction.
Anal Methods
September 2025
Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
This study introduces a new, highly sensitive, and reliable method for detecting and measuring orthophosphate in environmental water samples. This method combines cetyltrimethylammonium bromide (CTAB)-mediated coacervation extraction with digital image-based colorimetry, providing a robust and efficient approach for orthophosphate analysis. In this system, CTAB, a cationic surfactant, serves a dual role as both an ion-pairing agent and an extraction medium.
View Article and Find Full Text PDFComprehensive DFT and SCAPS-1D Study of Structural, Electronic, Optical, Mechanical, Phonon, Thermoelectric, and Photovoltaic Properties of Lead-Free ZBrO (Z = K, Rb, Cs, and Fr) Anti-Perovskites.
J Comput Chem
September 2025
Department of Electrical and Electronic Engineering, Begum Rokeya University, Rangpur, Bangladesh.
This study presents a comprehensive first-principles and device-performance investigation of alkali metal-based anti-perovskites ZBrO (Z = K, Rb, Cs, and Fr) for advanced optoelectronic and photovoltaic applications. Using density functional theory (DFT) with GGA-PBE and mGGA-rSCAN functionals, we analyzed the structural, electronic, optical, mechanical, phonon, population, and thermoelectric properties of these compounds. All ZBrO materials exhibit direct band gaps and strong optical absorption in the visible-UV spectrum.
View Article and Find Full Text PDFTeaching Occlusal Splints in the Digital Age: Comparing Student Experiences with Conventional and CAD/CAM Workflows.
J Dent Educ
September 2025
Departamento De Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Universidade De São Paulo, São Paulo, Brasil.
Background: The teaching of occlusal splint therapy in dental education is evolving with the integration of digital workflows. Although digital tools offer operational advantages, conventional methods remain pedagogically relevant. Understanding students' perceptions of both approaches is essential for guiding curriculum innovation.
View Article and Find Full Text PDFEarly itch relief with ivarmacitinib improves quality of life, working productivity, and sleep quality in patients with moderate-to-severe atopic dermatitis: a post hoc analysis of a phase III trial.
J Dermatolog Treat
December 2025
Department of Dermatology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Background: Ivarmacitinib (SHR0302), a selective Janus kinase-1 inhibitor, is a novel treatment for moderate-to-severe atopic dermatitis (AD).
Objectives: This post hoc analysis evaluated the impact of early itch relief with ivarmacitinib on quality of life (QoL), working productivity, and sleep quality in affected patients.
Methods: Data from ivarmacitinib treatment groups in a phase III trial (NCT04875169) were analyzed.
A Sensitive and Comprehensive LC-MS/MS Method for the Analysis of Hallucinogens, Synthetic Cathinones, and Synthetic Cannabinoids in Urine Samples.
J Mass Spectrom
October 2025
Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, Rio Grande do Sul, Brazil.
The laboratory analysis of new psychoactive substances and related drugs is crucial for accurate clinical and forensic diagnosis of poisonings. Given this, a new LC-MS/MS method for analyzing hallucinogens, synthetic cathinones, and synthetic cannabinoids in urine was developed. Urine samples were extracted using a liquid-liquid extraction protocol optimized via a multivariate experimental design.
View Article and Find Full Text PDF