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http://dx.doi.org/10.1038/s41422-024-00974-9 | DOI Listing |
Infect Drug Resist
September 2025
Department of Infection Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
This study presents a rare case of severe acute bacterial skin and soft tissue infection (ABSSSI) following freshwater fish spike injury in a 73-year-old man. Within 24 hours of sustaining the wound, the patient developed septic shock and progressive necrotizing fasciitis. Despite early administration of broad-spectrum antibiotics and intensive care, his condition deteriorated, necessitating below-the-elbow amputation on hospital day four.
View Article and Find Full Text PDFProg Nucl Magn Reson Spectrosc
September 2025
Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy. Electronic address:
Studying multidomain proteins, especially those combining well-folded domains with intrinsically disordered regions (IDRs), requires specific Nuclear Magnetic Resonance (NMR) techniques to address their structural complexity. To illustrate this, we focus here on the nucleocapsid protein from SARS-CoV-2, which includes both structured and disordered regions. We applied a suite of NMR methods, combining ARTINA software for automatic assignment and structure modelling with multi-receiver experiments that simultaneously capture signals from different nuclear spins, increasing both data quality and acquisition efficiency.
View Article and Find Full Text PDFSci Rep
September 2025
Department of Mechanical Engineering, College of Engineering, University of Ha'il, Ha'il City, 81451, Saudi Arabia.
Accurate assessment of intracranial aneurysm rupture risk, particularly in Middle Cerebral Artery (MCA) aneurysms, relies on a detailed understanding of patient-specific hemodynamic behavior. In this study, we present an integrated framework that combines Computational Fluid Dynamics (CFD) with Proper Orthogonal Decomposition (POD) and machine learning (ML) to efficiently model pulsatile blood flow using a Casson non-Newtonian fluid model, without incorporating fluid-structure interaction (FSI). Patient-specific vascular geometries were reconstructed from DICOM imaging data and simulated using ANSYS Fluent to capture key hemodynamic factors, including velocity components, pressure, wall shear stress (WSS), and oscillatory shear index (OSI).
View Article and Find Full Text PDFbioRxiv
August 2025
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
The RAS family of small GTPases are molecular switches that convey downstream signals regulating cell proliferation, differentiation, and apoptosis. The signaling competent GTP-bound RAS transitions to its inactive GDP-bound form through γ-phosphate hydrolysis. Oncogenic RAS mutations hamper GTP hydrolysis and are present in up to 30% of all human cancers.
View Article and Find Full Text PDFJ Med Imaging Radiat Sci
August 2025
Department of Radiology, School of Medicine, University of Health and Allied Sciences, Ho, Ghana.
Introduction: Access to timely image interpretation is critical for accurate diagnosis and effective patient management. However, radiologist shortages are likely to compromise image interpretation turnaround times (TATs) and leave many radiographs unreported. This cross-sectional survey-based study examines image interpretation TATs and the volume of unreported radiographs in Ghanaian healthcare facilities.
View Article and Find Full Text PDF