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Chocolate production faces nutritional, environmental and socio-economic challenges present in the conventional cocoa value chain. Here we developed an approach that addresses these challenges by repurposing the often-discarded pectin-rich cocoa pod endocarp and converting it into a gel. This is done using cocoa pulp juice concentrate to replace traditional sugar from sugar beets. Although swelling of fibres, proteins and starches can limit gel incorporation, our proposed chocolate formulation contains up to 20 wt% gel. It also has comparable sweet taste as traditional chocolate while offering improved nutritional value with higher fibre and reduced saturated fatty acid content. A cradle-to-factory life cycle assessment shows that large-scale production of this chocolate could reduce land use and global warming potential compared with average European dark chocolate production. The process also provides opportunities for diversification of farmers' income and technology transfer, offering potential socio-economic benefits for cocoa-producing regions.
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http://dx.doi.org/10.1038/s43016-024-00967-2 | DOI Listing |
Carbohydr Polym
November 2025
Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, NE1 8ST Newcastle Upon Tyne, United Kingdom; Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, 46022, Valencia, Spain. Electronic address: joel.l.g.hernandez@north
Polysaccharides, widely used in food, pharmaceutical and industrial sectors, are abundant in Theobroma species pod husk waste (T. cacao, T. grandiflorum and T.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
September 2025
Department of Exacts Science, State University of Santa Cruz, Ilheus, Bahia, 45662-900, Brazil.
Environmental pollution from toxic metals and waste generation have become major global concerns, driven by industrial and agricultural growth. To produce the adsorbent, the fungus Aspergillus niger ATCC 1004 was inoculated into cocoa pod husk bran. The samples were labeled as fermented solid (FS) and non-fermented solid (NFS) and characterized using X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, point of zero charge, and surface area analysis via N₂adsorption using the Brunauer-Emmett-Teller method.
View Article and Find Full Text PDFJ Environ Health Sci Eng
December 2025
Nitte (Deemed to be University), NMAM Institute of Technology (NMAMIT), Department of Biotechnology Engineering, Nitte, Karnataka 574110 India.
Unlabelled: Bioenergy plays a crucial role in addressing the global energy crisis. The utilization of agricultural byproducts for biofuel production through fermentation is well-established. Among various pretreatment methods, breaking lignin and cellulose bonds under heat and pressure to release sugar moieties is the most predominant approach.
View Article and Find Full Text PDFBMC Plant Biol
August 2025
Sustainable Perennial Crops Laboratory, Agricultural Research Service, Department of Agriculture, Beltsville, MD, 20705, USA.
Background: Cacao (Theobroma cacao L.) breeding and improvement rely on understanding germplasm diversity and trait architecture. This study characterized a cacao collection (173 accessions) evaluated in Puerto Rico, examining phenotypic diversity, trait interrelationships, and performing comparative analyses with published Trinidad and Colombia datasets.
View Article and Find Full Text PDFMicroorganisms
June 2025
Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Escuela Superior Politécnica del Litoral (ESPOL), Campus Gustavo Galindo, Km. 30.5 Vía Perimetral, Guayaquil P.O. Box 09-01-5863, Ecuador.
(MR, frosty pod rot) and (MP, witches' broom disease) pose critical threats to cacao production in Latin America. This study explores the biocontrol potential of spp. strains against these pathogens through exploratory analysis of mycoparasitism, chitinolytic activity, and volatile organic compound (VOC) production.
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