Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Unlike naturally derived peptides, computationally designed sequences offer programmed self-assembly and charge display. Herein, new tetrameric, coiled coil-forming peptides were computationally designed ranging from 8 to 29 amino acids in length. Experimental investigations revealed that only the sequences having three or more heptads (i.e., 21 or more amino acids) exhibited coiled coil behavior. The shortest stable coiled coil sequence had a melting temperature () of approximately 58 ± 1 °C, making it ideal for thermoreversible assembly over moderate temperatures. Effects of pH and monovalent salt were examined, revealing structural stability over a pH range of 4 to 11 and an enhancement in with the addition of salt. The incorporation of the coiled coil as a hydrogel cross-linker results in a thermally and mechanically reversible hydrogel. A subsequent demonstration of the hydrogel printed through a syringe illustrated one of many potential uses from 3D printing to injectable hydrogel drug delivery.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.biomac.4c00355 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Balloon inflation predicts recanalization of intracranial aneurysms treated with coiling alone.
Interv Neuroradiol
September 2025
Department of Neurological Surgery, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Lake Success, New York, USA.
BackgroundEndovascular coil embolization is a common treatment for intracranial aneurysms, but aneurysm recanalization remains a significant problem that may necessitate retreatment. This study aimed to identify patient, aneurysm, and procedural factors associated with recanalization in aneurysms treated exclusively with coil embolization.MethodsThis single center retrospective study assessed intracranial aneurysms treated with coiling-only between 2017 and 2022.
View Article and Find Full Text PDFTRIM28 is a target for paramyxovirus V proteins.
PLoS Pathog
September 2025
Institute of Medical Virology, University of Zurich, Zurich, Switzerland.
SUMO-modified Tripartite Motif Protein 28 (TRIM28; KAP1) plays a crucial role in repressing endogenous retroelement (ERE) transcription. We previously provided evidence that loss of SUMO-modified TRIM28 triggered by influenza A virus (IAV) infection promotes activation of host antiviral immunity via a mechanism involving derepression of EREs and production of immunostimulatory RNAs. While the IAV NS1 protein might limit consequences of such activation via its dsRNA-binding activity, we hypothesized that other human pathogenic viruses could have evolved more direct strategies to counteract this potential ERE-based defense system.
View Article and Find Full Text PDFHow to use the nautilus intrasaccular system.
Interv Neuroradiol
September 2025
Department of Neuroradiology, Queen's Hospital, Greater London, UK.
The Nautilus intrasaccular system (EndoStream Medical, Israel) is a spiral-shaped neck-bridging endovascular device designed to support coiling of intracranial aneurysms [1-3]. It is deployed into the aneurysm sac through a 0.0165" or 0.
View Article and Find Full Text PDFEngineering a Coiled-Coil Protein for DARPin Presentation as a Potent SARS-CoV-2 Therapeutic.
Biomacromolecules
September 2025
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 950 Atlantic Dr NW, Atlanta, Georgia 30332, United States.
The COVID-19 pandemic has demonstrated the need for rapid, flexible, and readily adaptable treatment options for future pandemic preparedness. Due to the speed at which viruses like SARS-CoV-2 mutate, the customary approach of using highly specific monoclonal antibodies as neutralization therapies is challenging, given their size, production complexity, and cost. Here, we leveraged rational protein design to create fusion proteins from small, antibody-mimetic proteins, Designed Ankyrin Repeat Proteins (DARPins) and a self-assembling hexameric coiled coil (CC-HEX).
View Article and Find Full Text PDFTurn-Over Technique Using a Balloon Guiding Catheter for Stable Transfemoral Neuroendovascular Therapy in Type III Aortic Arch with Severe Vessel Tortuosity.
J Neuroendovasc Ther
August 2025
Department of Neurosurgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima, Japan.
Objective: Endovascular treatment of cerebrovascular diseases can be challenging in patients with difficult access routes. We describe a turn-over technique using a balloon guiding catheter (BGC) via the transfemoral approach to perform neuroendovascular treatment.
Case Presentation: An 80-year-old female with a recurrent right middle cerebral artery (MCA) aneurysm after coil embolization underwent successful stent-assisted coiling via the transfemoral approach.