Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor.

ACS Appl Mater Interfaces

Key Laboratory of Optoelectronic Materials and Devices, Chinese Academy of Sciences, Beijing 100083, China.

Published: May 2024


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Exosomes are becoming more widely acknowledged as significant circulating indicators for the prognosis and diagnosis of cancer. Circulating exosomes are essential to the development and spread of cancer, according to a growing body of research. Using existing technology, characterizing exosomes is quite difficult. Therefore, a direct, sensitive, and targeted approach to exosome detection will aid in illness diagnosis and prognosis. The review discusses the new strategies for exosome isolation and detection technologies from microfluidic chips to nanoplasmonic biosensors, analyzing the advantages and limitations of these new technologies. This review serves researchers to better understand exosome isolation and detection methods and to help develop better exosome isolating and detecting devices for clinical applications.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.3c19396DOI Listing

Publication Analysis

Top Keywords

exosome isolation
12
isolation detection
12
microfluidic chips
8
chips nanoplasmonic
8
exosome
5
detection
4
detection microfluidic
4
nanoplasmonic biosensor
4
biosensor exosomes
4
exosomes acknowledged
4

Similar Publications

Silencing CD151 Gene in Donor Triple-Negative Breast Cancer Cells Attenuates Exosome-Driven Functions of Recipient Cells.

Exp Cell Res

September 2025

Cancer Biology Laboratory, Dept of Life Sciences, GITAM School of Sciences, GITAM (Deemed to be University), Visakhapatnam-530045, Andhra Pradesh, India. Electronic address:

CD151 is a tetraspanin, abnormally expressed in triple negative breast cancer (TNBC). It is a prominent component of exosomes, facilitating the secretion of proteins that promote metastasis and drug resistance. We have previously demonstrated that silencing the CD151 gene reduces metastasis in TNBC.

View Article and Find Full Text PDF

Myostatin knockout mice muscle derived exosome inhibited dexamethasone-induced muscle atrophy.

Int Immunopharmacol

September 2025

Department of Animal Science, College of Agricultural, Yanbian University, Yanji 133002, China; Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji 133002, China. Electronic address:

Objective: Long-term administration of dexamethasone (DEX) to treat severe inflammation or autoimmune disorders often result in skeletal muscle atrophy and functional decline. Exosomes facilitate intercellular communication by transferring bioactive molecules, reflecting the characteristics of their tissue of origin. Myostatin-knockout (MSTN) mice exhibit muscle hypertrophy, and their muscle-derived exosomes (KO-EXOs) retain this phenotype.

View Article and Find Full Text PDF

Identification of poor prognostic factors using circulating extracellular vesicles in durvalumab consolidation therapy for locally advanced non-small cell lung cancer.

Lung Cancer

September 2025

Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo, Japan; Division of Next-Generation Drug Development Research, Research Center for Medical Sciences, The Jikei University School of Medicine, 3-25-8 Ni

Background: The risk factors associated with treatment resistance to consolidation durvalumab following chemoradiotherapy (CRT) for locally advanced non-small cell lung cancer (NSCLC) have not been well established.

Methods: Extracellular vesicles (EVs) were isolated from the pretreatment serum of 73 patients treated with consolidation durvalumab. Isolation was performed using CD9/CD63 antibodies, and EV proteins were identified using liquid chromatography-tandem mass spectrometry (LC-MS).

View Article and Find Full Text PDF

Background: Sarcomas are rare cancer with a heterogeneous group of tumors. They affect both genders across all age groups and present significant heterogeneity, with more than 70 histological subtypes. Despite tailored treatments, the high metastatic potential of sarcomas remains a major factor in poor patient survival, as metastasis is often the leading cause of death.

View Article and Find Full Text PDF

Exosomal Proteome from Hepatocellular Carcinoma Patient-Derived Xenograft Mice Serves as Identity of Liver Cancer.

J Proteome Res

September 2025

State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing 102206, China.

Hepatocellular carcinoma (HCC) constitutes approximately 90% of liver cancers, yet its early detection remains challenging due to the low sensitivity of current diagnostic methods and the difficulty in identifying minimal cancer cells within the body. This study employed a patient-derived xenograft (PDX) mouse model to screen for biomarkers, leveraging its advantage of low background interference compared to human serum exosome studies. Using a novel microextraction technique, exosomes were isolated from just one microliter of serum from HCC PDX mice, followed by proteomic profiling.

View Article and Find Full Text PDF