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Article Abstract
Background: Up to 20% of people with HIV (PWH) fail to recover their CD4+ cell counts to levels similar to healthy controls after suppressive antiretroviral therapy (ART). Immune non-responders (INRs) are PWH on suppressive ART with CD4+ cell counts lower than 350 cells/mL, whereas their CD8+ cell counts are higher than healthy controls. We are the first group to report that increased anti-CD4 autoantibody IgGs in INRs are responsible for blunted CD4+ cell reconstitution in PWH with ART and viral suppression through anti-CD4 IgG-induced antibody-mediated cytotoxicity (ADCC) against CD4+ cells . Notably, anti-CD4 IgG-mediated poor CD4+ cell recovery from suppressive ART is the only mechanism targeting CD4+ cells, specifically.
Results: We provide a detailed one-by-one step protocol from antigen-specific antibody isolation using plasma samples, to ADCC assay.
Conclusions: To promote reproducible research, a detailed protocol for isolating anti-CD4 IgG autoantibodies from plasma samples of PWH and evaluating ADCC effects is reported here.•Antigen-specific antibody isolation using human plasma samples•Antibody-mediated cytotoxicity (ADCC).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033182 | PMC |
| http://dx.doi.org/10.1016/j.mex.2024.102698 | DOI Listing |
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