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Article Abstract
Eucalyptus scab and shoot malformation caused by is an emerging disease and a serious threat to the global commercial forestry industry. The disease was first discovered in North Sumatra, Indonesia, and now requires a simple and effective method for early pathogen detection. In this study, a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed for . A unique region in a secondary metabolite gene cluster was used as a target for the assay. To test robustness of the assay, LAMP was verified in 15 strains of . A specificity test against 23 closely related species and three fungal species commonly isolated on showed that the LAMP assay exclusively identified isolates. The assay had a high level of sensitivity, able to detect 0.01 ng (approximately 400 target copies) of pure DNA. Furthermore, using a simple DNA extraction method, it was possible to use this assay to detect in infected leaves.
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