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[This corrects the article DOI: 10.1371/journal.pone.0261548.].
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Front Cell Infect Microbiol
September 2025
Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Hradec Kralove, Czechia.
Background: Chronic vulvovaginal discomfort (CVD) is a complex syndrome with many unresolved questions regarding its etiology, diagnosis, and management in relation to the vaginal microbiota.
Methods: Cervicovaginal fluid of 91 CVD patients and 35 healthy controls was obtained at the beginning and end of the follow-up period. The bacterial community state types (CST) in the vagina were assessed using next-generation sequencing (NGS).
Cytopathology
August 2025
Department of Pathology & Laboratory Medicine, University of Kentucky College of Medicine, Lexington, Kentucky, USA.
Background: Thyroid tumours occur in patients with Birt-Hogg-Dubé syndrome (BHD), a condition caused by germline alterations in the FLCN gene that confers an increased cancer risk. Somatic FLCN mutations may also contribute to tumorigenesis. In this study, we present the first description of the cytopathology of two thyroid tumours evaluated by fine-needle aspiration (FNA) and harbouring pathogenic FLCN mutations.
View Article and Find Full Text PDFBackground: Next generation sequencing (NGS) of cell free tumour DNA (ctDNA) provides a snapshot of lymphoma mutations correlating with tumour burden. We evaluated the ability of ctDNA to molecularly profile and track disease burden in patients with aggressive-B-cell lymphoma.
Methods: Patients were prospectively recruited from the 'standard-of-care' clinic based on high-risk clinical features.
Microbiol Spectr
August 2025
Department of Precision Medical Center, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China.
Unlabelled: Integration of high-risk human papillomavirus (hrHPV) DNA is a critical event in carcinogenesis and a promising biomarker for risk stratification. However, the high cost of next-generation sequencing (NGS) limits its widespread clinical adoption. We developed HPVPool-Seq, an innovative pooling strategy that leverages the inherent diversity of HPV genotypes as natural barcodes, enabling cost-effective, scalable integration detection.
View Article and Find Full Text PDFEnviron Mol Mutagen
August 2025
Division of Genetic and Molecular Toxicology, U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas, USA.
The ability to predict rodent lifetime tumor responses from short-term exposures and a scientific basis for rodent to human extrapolation are unmet needs in cancer risk assessment. To address these needs, quantitation of cancer driver mutations (CDMs) was integrated with an error-corrected, next generation sequencing (NGS) approach. The method developed, CarcSeq, involves performing multiple, high-fidelity PCR reactions to amplify hotspot CDM-containing target sequences, tagging amplicons with 9 base unique identifier sequences, and constructing libraries from the pooled amplicons.
View Article and Find Full Text PDF