Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Celiac Disease (CeD) is an autoimmune disorder with various symptoms upon gluten exposure. Dendritic cells (DCs) play a crucial role in gliadin-induced inflammation. Vitamin A (retinol; Ret) and its metabolite, retinoic acid (RA), along with tryptophan (Trp) and its metabolite, kynurenic acid (KYNA), are known to influence the immune function of DCs and enhance their tolerogenicity. This research aims to assess the impact of gliadin on DC maturation and the potential of vitamin A and tryptophan to induce immune tolerance in DCs. The monocyte cells obtained from peripheral blood mononuclear cells (PBMCs) of celiac disease patients were differentiated into DCs in the absence or presence of Ret, RA, Trp, KYNA, and then stimulated with peptic and tryptic (PT) digested of gliadin. We used flow cytometry to analyze CD11c, CD14, HLA-DR, CD83, CD86, and CD103 expression. ELISA was carried out to measure TGF-β, IL-10, IL-12, and TNF-α levels. qRT-PCR was used to assess the mRNA expression of retinaldehyde dehydrogenase 2 (RALDH2) and integrin αE (CD103). The mRNA and protein levels of Indoleamine 2, 3-dioxygenase (IDO) was analyzed by qRT-PCR and Western blot assays, respectively. Our findings demonstrate that PT-gliadin enhances the expression of maturation markers, i.e. CD83, CD86 and HLA-DR and promote the secretion of TNF-α and IL-12 in DCs. Interestingly, vitamin A, tryptophan, and their metabolites increase the expression of CD103, while limiting the expression of HLA-DR, CD83, and CD86. They also enhance RALDH2 and IDO expression and promote the secretion of TGF-β and IL-10, while limiting IL-12 and TNF-α secretion. These findings suggest that vitamin A and tryptophan have beneficial effects on PT-gliadin-stimulated DCs, highlighting their potential as therapeutic agents for celiac disease. However, further research is needed to fully understand their underlying mechanisms of action in these cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10753-024-02004-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
HIV-induced gut microbiota dysbiosis perpetuates mucosal barrier disruption and systemic inflammation despite antiretroviral therapy (ART), creating a tumor-permissive microenvironment. This review synthesizes evidence linking HIV-associated microbial alterations to oncogenesis through three convergent metabolic axes: (1) butyrate deficiency impairing epithelial energy metabolism and anti-tumor immunity; (2) tryptophan metabolism dysregulation compromising gut barrier integrity via depletion and -mediated phenylethylamine overproduction; and (3) vitamin B biosynthesis defects disrupting DNA repair and Th1/Th2 balance. Comparative profiling across HIV-associated malignancies-non-Hodgkin lymphoma, cervical cancer, hepatocellular carcinoma, and lung cancer-reveals conserved dysbiotic signatures: depletion of anti-inflammatory taxa (, ) and expansion of pro-inflammatory genera (, ).
View Article and Find Full Text PDFThe multilayered regulation of aromatic amino acid biosynthesis in plants.
Trends Biochem Sci
August 2025
Department of Botany, University of Wisconsin, Madison, WI 53706, USA. Electronic address:
The shikimate and aromatic amino acid (AAA) biosynthetic pathways are crucial for the production of L-phenylalanine (Phe), L-tyrosine (Tyr), and L-tryptophan (Trp), as well as vitamins, hormones, and an array of plant natural products, including lignin, a major reservoir of organic carbon on Earth. In this review, we summarize recent advances in the mechanisms that dynamically regulate the AAA biosynthetic pathways of plants, with a particular focus on Phe biosynthesis due to its central role as a precursor to phenylpropanoids. The integration of AAA biosynthesis with upstream and downstream plant metabolism is also discussed, as well as how this fundamental knowledge can inform the bioengineering of plant-based platforms for sustainable production of AAA-derived natural products.
View Article and Find Full Text PDFDietary protein sources in concentrate supplementation influence growth performance by manipulating gut microbiota and serum metabolites in suckling Donkey foals.
Anim Microbiome
August 2025
Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co- construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science, Shandong Agricultural University, Ta
Background: Protein is a primary nutrient in concentrate supplementation for donkey foals, and the source of this protein significantly influences their growth and development. Milk-derived protein sources, such as milk powder, casein, and whey protein, are widely used in milk replacers for donkey foals due to their balanced nutritional profiles, high digestibility, and high bioavailability. However, the increasing costs of milk powder and whey protein have prompted researchers to explore alternative protein sources, with soy protein being a particularly promising option.
View Article and Find Full Text PDFTranscriptomic and proteomic insights into the growth-promoting mechanisms of dietary γ-aminobutyric acid (GABA) in tawny puffer (Takifugu flavidus).
Comp Biochem Physiol Part D Genomics Proteomics
August 2025
Shanghai Fisheries Research Institute, Shanghai 200433, China.
γ-aminobutyric acid (GABA) has been shown to promote growth in certain aquatic species, yet the molecular mechanisms remain unclear. This study investigated the effects of GABA in juvenile tawny puffer (Takifugu flavidus) via growth performance, liver transcriptomic and proteomic analyses, and plasma stress indices. Dietary supplementation with 100 mg/kg GABA for 50 days significantly improved weight gain rate (WGR) and specific growth rate (SGR), alongside higher feeding rate (FR) and hepatosomatic index (HSI).
View Article and Find Full Text PDFEfficacy of a Preparation based on Symbiotic Association Between Inulin, FOS, L. rhamnosus GG, Bromelin, Boswellia, Vitamin D3, Quercetin and L-tryptophan in Mild-to-Moderate Ulcerative Colitis: A Pilot Retrospective Multicenter Study.
Rev Recent Clin Trials
August 2025
Territorial Gastroenterology Service, ASL BAT, Andria, Italy.
Background And Objectives: Several compounds based on short-chain fatty acids and/or probiotics/prebiotics have shown promising results in the therapy of mild-to-moderate ulcerative colitis (UC). The aim of the present study is to investigate the effectiveness of a preparation based on symbiotic association between inulin, fructooligosaccharides (FOS), Lactobacillus rhamnosus GG, bromelin, Boswellia, vitamin D3, quercetin and L-tryptophanon in patients with active mild-to-moderate UC.
Materials: andMethods: This was a multicentre, retrospective, observational cohort study between January 2023 and June 2023.