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Article Abstract
Background: Mesenchymal-epithelial transition () amplification is a crucial oncogenic driver and a resistance mechanism to epidermal growth factor receptor tyrosine kinase inhibitors (TKIs) of non-small-cell lung cancer (NSCLC). Fluorescence hybridization (FISH) is the gold standard for amplification detection. However, it is inapplicable when tissue samples are unavailable.
Objective: This study assessed the performance of plasma droplet digital polymerase chain reaction (ddPCR) in amplification detection in NSCLC patients.
Design And Methods: A total of 87 NSCLC patients were enrolled, and 94 paired tissue and plasma samples were analyzed for the concordance between FISH and plasma ddPCR/tissue next-generation sequencing (NGS) in detecting amplification. In addition, the efficacy of patients with amplification using different detection methods who were treated with MET-TKIs was evaluated.
Results: Plasma ddPCR showed substantial concordance with FISH (74.1% sensitivity, 92.5% specificity, and 87.2% accuracy with a kappa value of 0.68) and outperformed tissue NGS (kappa value of 0.64) in amplification detection. Combined plasma ddPCR and tissue NGS showed substantial concordance with FISH (92.3% sensitivity, 89.2% specificity, and an accuracy of 90.1% with a kappa value of 0.77). The efficacy is comparable in these NSCLC patients with amplification detected by FISH and plasma ddPCR who were treated with MET-TKIs.
Conclusion: Plasma ddPCR is a potentially reliable method for detecting amplification in advanced NSCLC patients. Combined plasma ddPCR and tissue NGS might be an alternative or complementary method to amplification detection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851729 | PMC |
| http://dx.doi.org/10.1177/17588359241229435 | DOI Listing |
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