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To celebrate the 50th anniversary of Cell Press and the Cell focus issue on structural biology, we discussed with scientists working across diverse fields how AlphaFold has changed their research and brought structural biology to the masses.
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http://dx.doi.org/10.1016/j.molcel.2023.12.035 | DOI Listing |
Top Curr Chem (Cham)
September 2025
Center for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, 27272, Sharjah, United Arab Emirates.
Controlling the size of gold nanoparticles (AuNPs) has been critical in diagnostics, biomolecular sensing, targeted therapy, wastewater treatment, catalysis, and sensing applications. Ultrasmall AuNPs (uAuNPs), with sizes Ranging from 2 to 5 nm, and gold nanoclusters (AuNCs), with sizes less than 2 nm, are often dealt with interchangeably in the literature, making it challenging to review them separately. Although they are grouped in our discussion, their chemical and physical properties differ significantly, partly due to their electronic properties.
View Article and Find Full Text PDFJ Med Chem
September 2025
Repare Therapeutics, 7171 Frederick-Banting, Building 2, H4S 1Z9 Montréal, Québec, Canada.
DNA polymerase theta (Polθ) plays a critical role in repairing DNA double-strand breaks through microhomology-mediated end joining (MMEJ) and has emerged as a key synthetic lethal drug target in cancers with homologous recombination (HR) deficiencies. Its inhibition has shown a strong potential to synergize with PARP inhibitors, particularly in tumors with deleterious or mutations. Here, we describe the discovery and preclinical development of RP-2119, a selective, potent, and bioavailable Polθ ATPase inhibitor.
View Article and Find Full Text PDFJ Cell Biol
November 2025
Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Two major protein recycling pathways have emerged as key regulators of enduring forms of synaptic plasticity, such as long-term potentiation (LTP), yet how these pathways are recruited during plasticity is unknown. Phosphatidylinositol-3-phosphate (PI(3)P) is a key regulator of endosomal trafficking and alterations in this lipid have been linked to neurodegeneration. Here, using primary hippocampal neurons, we demonstrate dynamic PI(3)P synthesis during chemical induction of LTP (cLTP), which drives coordinate recruitment of the SNX17-Retriever and SNX27-Retromer pathways to endosomes and synaptic sites.
View Article and Find Full Text PDFElife
September 2025
Graduate School of Life Science, Hokkaido University, Sapporo, Japan.
DNA replication requires recruitment of Cdc45 and GINS into the MCM double hexamer by initiation factors to form an active helicase, the Cdc45-MCM-GINS (CMG) complex, at the replication origins. The initiation factor Sld3 is a central regulator of Cdc45 and GINS recruitment, working with Sld7 together. However, the mechanism through which Sld3 regulates CMG complex formation remains unclear.
View Article and Find Full Text PDFmSystems
September 2025
Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Genome-scale metabolic models (GEMs) are widely used in systems biology to investigate metabolism and predict perturbation responses. Automatic GEM reconstruction tools generate GEMs with different properties and predictive capacities for the same organism. Since different models can excel at different tasks, combining them can increase metabolic network certainty and enhance model performance.
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