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X-ray diffusive dark-field imaging, which allows spatially unresolved microstructure to be mapped across a sample, is an increasingly popular tool in an array of settings. Here, we present a new algorithm for phase and dark-field computed tomography based on the x-ray Fokker-Planck equation. Needing only a coherent x-ray source, sample, and detector, our propagation-based algorithm can map the sample density and dark-field/diffusion properties of the sample in 3D. Importantly, incorporating dark-field information in the density reconstruction process enables a higher spatial resolution reconstruction than possible with previous propagation-based approaches. Two sample exposures at each projection angle are sufficient for the successful reconstruction of both the sample density and dark-field Fokker-Planck diffusion coefficients. We anticipate that the proposed algorithm may be of benefit in biomedical imaging and industrial settings.
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http://dx.doi.org/10.1364/OE.509604 | DOI Listing |
Cureus
September 2025
Department of Urology, Gunma University School of Medicine, Maebashi, JPN.
Syphilis cases are increasing in Japan, highlighting the urgent need for rapid and accurate diagnosis. In primary syphilis, serological tests may yield negative results, so direct microscopic observation of remains an important but often underused diagnostic method. We present a simple, widely accessible phase-contrast microscopy system, featuring a USB camera and monitor, enabling high-clarity, real-time observation of live wild-type treponemes directly from patient lesions.
View Article and Find Full Text PDFJ Microsc
August 2025
Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel.
The contributions of coherent bright-field phase and incoherent dark-field amplitude contrast are investigated for thick biological specimens. A model for a T4 phage is constructed and images simulated for both TEM and STEM phase contrast using a multislice code. For TEM, the fraction of the illumination intensity available for phase contrast imaging is limited by the fraction of electrons in the zero loss peak, the plasmon peak, or the Landau distribution peak for very thick specimens.
View Article and Find Full Text PDFPhys Chem Chem Phys
August 2025
AREA Science Park, 34149 Trieste, Italy.
Rare-earth nickelates, such as LaNiO (LNO), exhibit complex electronic properties, with ordered oxygen vacancies (OOV) influencing conductivity and magnetic behavior. We investigate the structural stability of strain-induced OOV phases in LNO thin films grown on SrTiO substrates and the impact of Ruddlesden-Popper (RP) faults. Using high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) and integrated differential phase contrast (iDPC) STEM imaging, we conducted atomic-scale structural and compositional analyses of OOV.
View Article and Find Full Text PDFEdge illumination (EI) is an established X-ray phase-contrast imaging method that relies on gratings to obtain attenuation, differential phase, and dark field contrast. Conventional gratings with one-dimensional line apertures, however, pose a major limitation in geometric flexibility of current EI-setups. That is, the gratings are designed for a fixed magnification and the period and aperture size of the gratings determine the fixed resolution.
View Article and Find Full Text PDFMicrosc Res Tech
August 2025
Applied Photonics and Nanophotonics Laboratory, Department of Physics, Tezpur University, Tezpur, India.
The rapid advancements in smartphone camera features and accessibility have garnered significant attention for smartphone-based imaging systems in recent years. However, due to variations in dimensions and camera positions across different phones, they often suffer from compatibility issues. In the present work, these issues have been addressed by using a Raspberry Pi single-board computer (SBC) as the platform for the development of a multimodal microscopic system.
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