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Study of the physiological effects of microgravity on humans is limited to non-invasive testing of astronauts. Microphysiological models of human organs recapitulate many functions and disease states. Here we describe the development of an advanced, semi-autonomous hardware platform to support kidney microphysiological model experiments in microgravity.
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http://dx.doi.org/10.21203/rs.3.rs-3750478/v1 | DOI Listing |
Biotechnol Bioeng
July 2025
Department of Biology, University of British Columbia, Kelowna, BC, Canada.
Three-dimensional (3D) in vitro cell culture models have revolutionized biomedical research by mimicking the complex 3D in vivo environment in the human body. Different types of 3D models have been established, including heterotypic systems such as, organ-on-a-chips which have been further developed into multiorgan-on-chip systems that simulate or mimic the mutual and multiplex physiological communication between (distant) organs that may not be physically connected with each other known as multiorgan crosstalk/interactions. These multiorgan interactions have been shown to be mediated by various factors including cells, soluble mediators (growth factors, cytokines etc.
View Article and Find Full Text PDFWe evaluated the impact of spaceflight on a microphysiologic model of calcium oxalate (CaOx) kidney stone disease. Proximal tubule epithelial cells cultured as confluent microtubules were exposed to CaOx crystals with or without potassium citrate (a potential countermeasure) to determine impact on gene expression. Nine genes were differentially expressed in response to CaOx crystal exposure during spaceflight.
View Article and Find Full Text PDFAdv Biol (Weinh)
July 2025
Sorbonne Université, Inserm, Common and Rare Kidney Diseases: from Molecular Events to Precision Medicine, CoRaKiD, Paris, F-75020, France.
Chronic kidney disease (CKD) affects over 850 million individuals worldwide, often progressing to stages requiring dialysis or kidney transplants. Central to kidney function is the glomerular filtration barrier (GFB), which selectively filters waste while retaining essential proteins. Traditional models, including animal studies and 2D cell cultures, fail to fully replicate the GFB's complexity, limiting CKD research.
View Article and Find Full Text PDFAdv Healthc Mater
June 2025
Department of Materials, Design and Manufacturing Engineering, School of Engineering, University of Liverpool, Liverpool, L69, UK.
The global increase in chronic diseases and comorbidities due to aging populations is placing significant strain on healthcare systems. Chronic kidney disease (CKD) and osteoarthritis (OA) are among the most prevalent conditions in the elderly, with complex interconnections driven by shared risk factors such as systemic inflammation and metabolic dysregulation. Despite the critical need to understand these interactions, traditional animal models often fail to adequately capture the multidirectional crosstalk between human comorbid conditions, limiting insights into their mechanisms and complicating translational research.
View Article and Find Full Text PDFExpert Opin Drug Saf
July 2025
Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA.
Introduction: Kidney diseases, including chronic kidney disease and acute kidney injury, pose major global health challenges due to their high prevalence and impact on morbidity and mortality. Despite medical advances, there remains an urgent need for improved drug development and therapeutic strategies to treat these conditions.
Areas Covered: This review examines how renal transporters influence drug handling, highlighting the impact of altered transporter function on toxin accumulation, organ injury, and systemic toxicity.