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Aerosol particles with rare specific properties act as nuclei for ice formation. The presence of ice nucleating particles in the atmosphere leads to heterogeneous freezing at warm temperatures and thus these particles play an important role in modulating microphysical properties of clouds. This work presents an ice nucleation cold stage instrument for measuring the concentration of ice nucleating particles in liquids. The cost is ∼ $10 k including an external chiller. Using a lower cost heat sink reduces the cost to ∼ $6 k. The instrument is suitable for studying ambient ice nucleating particle concentrations and laboratory-based process-level studies of ice nucleation. The design plans allow individuals to self-manufacture the cold-stage using 3D printing, off-the-shelf parts, and a handful of standard tools. Software to operate the instrument and analyze the data is also provided. The design is intended to be simple enough that a graduate student can build it as part of a course or thesis project. Costs are kept to a minimum to facilitate use in classroom demonstrations and laboratory classes.
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http://dx.doi.org/10.1016/j.ohx.2023.e00491 | DOI Listing |
ACS Appl Mater Interfaces
September 2025
Department of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
Cyclic peptides (CPs) are versatile building blocks whose conformational constraints foster ordered supramolecular architectures with potential in biomedicine, nanoelectronics, and catalysis. Herein, we report the development of biomimetic antifreeze materials by conjugating CPs bearing ice-binding residues to 4-arm polyethylene glycol (PEG) via click chemistry. The concentration-dependent self-assembly of these CP-PEG conjugates induces programmable morphological transitions, forming nanotube networks above the critical aggregation concentration (CAC) and two-dimensional nanosheet networks near the CAC.
View Article and Find Full Text PDFJ Chem Phys
September 2025
Dipartimento di Fisica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, Rome 00185, Italy.
The equilibration dynamics of ultrastable glasses subjected to heating protocols has attracted recent experimental and theoretical interest. With simulations of the mW water model, we investigate the devitrification and "melting" dynamics of both conventional quenched (QG) and vapor deposited (DG) amorphous ices under controlled heating ramps. By developing an algorithm to reconstruct hydrogen-bond networks, we show that bond ring statistics correlate with the structural stability of the glasses and allow tracking crystalline and liquid clusters during devitrification and melting.
View Article and Find Full Text PDFNanoscale
September 2025
School of Mechanical Engineering, Shandong University of Technology, Zibo 255000, China.
Metal matrix composites are widely employed in aerospace and marine engineering due to their excellent mechanical properties and chemical stability. However, their surfaces remain vulnerable to corrosion, icing, and mechanical wear, severely compromising long-term reliability in harsh environments. Inspired by natural superhydrophobic surfaces such as lotus leaves, functional interfaces with high water repellency and interfacial stability can be engineered through the synergistic design of hierarchical micro/nanostructures and low-surface-energy chemical modifications.
View Article and Find Full Text PDFJ Therm Biol
August 2025
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada; Innovation and Portfolio Management, Canadian Blood Services, Edmonton, AB, Canada. Electronic address:
Extreme temperature fluctuations during routine handling and shipping of cryopreserved cell products significantly compromise product quality in ways that extend beyond the duration and peak temperature of the fluctuation. The type of cryoprotectant used and the initial ice nucleation temperature influence ice crystal growth during rewarming events, in turn impacting cell survival. Using a cryomicroscope together with temperature profiles recorded in cord-blood units, ice crystal growth was tracked through five transient-warming events (TWEs) that peaked at -30 °C, -20 °C, or -10 °C.
View Article and Find Full Text PDFCryobiology
September 2025
UCIBIO - Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal. Electroni
High-throughput experimental screening is desirable to minimize data acquisition time from vast workloads. Cell cryopreservation experiments are routinely performed in single-sample cryovials despite cell seeding being performed in 96-well microplates because these substrates are known to induce microliter supercooling, are prone to thermal compressibility and their lengthy preparation period extends cell exposure time to potentially cytotoxic cryoprotectants. Rather than improving the methodological preciseness of cooling, latest efforts have focused on refining cryoprotectant formulations and supplement precautionary ice nucleators.
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