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Key Clinical Message: EDTA-dependent pseudothrombocytopenia as well as myelosuppression should be suspected when thrombocytopenia occurs in patients with autoimmune disease during chemotherapy.
Abstract: A patient with pancreatic cancer and ulcerative colitis developed transient ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia with exacerbation of ulcerative colitis during chemotherapy. Unfortunately, pseudothrombocytopenia could not be immediately detected because thrombocytopenia was masked by a reasonable time course of adverse events associated with chemotherapy and ulcerative colitis recurrence. When thrombocytopenia occurs during chemotherapy, especially in patients with autoimmune diseases, EDTA-dependent pseudothrombocytopenia and bone marrow suppression caused by anti-cancer agents should be suspected.
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http://dx.doi.org/10.1002/ccr3.8153 | DOI Listing |
Cureus
March 2025
NICM Health Research Institute, Western Sydney University, Westmead, AUS.
Ethylenediaminetetraacetic acid-induced pseudo-thrombocytopenia (EDTA-PTCP) is an artefact caused by EDTA-dependent platelet clumping in laboratory testing. Unrecognized cases may lead to unnecessary investigations, diagnostic confusion, and inappropriate medical management. We present a case of EDTA-PTCP, a condition that is still commonly overlooked in clinical practice.
View Article and Find Full Text PDFPeerJ
May 2025
Department of Laboratory Medicine, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, Jiangsu, China.
Background: EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is an phenomenon that may lead to expensive, time-consuming, and invasive diagnostic procedures as well as unnecessary patient treatment. The purpose of this study was to explore the effects of time, anticoagulant and detection channel on the platelet (PLT) count of EDTA-PTCP samples, and to suggest a better method for correcting spurious low PLT counts.
Methods: In this study, 43 identified EDTA-PTCP samples were collected.
Turk J Pediatr
November 2024
Department of Pediatric Hematology, Faculty of Medicine, Gazi University, Ankara, Türkiye.
Background: Pseudothrombocytopenia is a spurious thrombocytopenia caused mostly by ethylenediaminetetraacetic acid (EDTA) use, and if detected early, unnecessary testing and treatment can be avoided. We present pseudothrombocytopenia caused by EDTA and citrate in an asymptomatic healthy child, as well as the value of using peripheral blood smear, platelet histogram, and flag data.
Case: A previously healthy 13-year-old girl with thrombocytopenia who developed tonsillitis 12 days previously was referred to our hematology department.
Open Vet J
May 2024
Faculty of Veterinary Science, Prince of Songkla University, Songkhla, Thailand.
Background: Pseudothrombocytopenia is a commonly obtained false negative result when analyzing feline platelet (PLT) count by an automated machine. It is related to ethylenediamine tetra-acetic acid (EDTA), a widely utilized anticoagulant in blood collection tubes, resulting in EDTA-dependent pseudothrombocytopenia (EDTA-PTCP).
Aim: To investigate whether treated with kanamycin enhanced the quantity of PLT aggregations in feline blood specimens collected using EDTA-PTCP.
Clin Chim Acta
March 2024
Department of Laboratory Medicine, Huashan Hospital Fudan University, Shanghai 200040, China. Electronic address:
Background: This study investigated the performance of the MC-100i, a pre-commercial digital morphology analyzer utilizing a convolutional neural network algorithm, in a multicentric setting involving up to 11 tertiary hospitals in China.
Methods: Blood smears were analyzed by MC-100i, verified by morphologists, and manually differentiated. The classification performance on WBCs and RBCs was evaluated by comparing the classification results using different methods.