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Article Abstract
Microtubules are essential components of eukaryotic cells. Myriad proteins associate with microtubules to facilitate the organization and operation of microtubule arrays. Various M icrotubule A ssociated P roteins (MAPs) assist the assembly and function of mitotic spindles and interphase arrays. Nine MAP65 genes exist in the genome of the acentrosomal model plant, and the function of majority of these proteins is unclear. To address this knowledge gap, we demonstrate the localization of MAP65-6 and MAP65-7 fusion proteins expressed from native promoters in interphase cells of developing seedlings. Analyses of these fusion proteins co-expressed with alpha-tubulin 6 reporters indicate that MAP65-6 and MAP65-7 bind a subset of interphase microtubules. Co-expression of GFP: MAP65-6 with mCherry: MAP65-2 from native promoters in showed overlapping localization patterns on interphase microtubule bundles. Collectively, these data suggested that MAP65-2 , -6, and -7 bind cortical microtubule bundles in plant interphase microtubule arrays.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630750 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000971 | DOI Listing |
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