Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Inflammation in the lungs and other vital organs in COVID-19 is characterized by the presence of neutrophils and a high concentration of neutrophil extracellular traps (NETs), which seems to mediate host tissue damage. However, it is not known whether NETs could have virucidal activity against SARS-CoV-2.
Methods: We investigated whether NETs could prevent SARS-CoV-2 replication in neutrophils and epithelial cells and what the consequence of NETs degradation would be in K18-humanized ACE2 transgenic mice infected with SARS-CoV-2.
Results: Here, by immunofluorescence microscopy, we observed that viral particles colocalize with NETs in neutrophils isolated from patients with COVID-19 or healthy individuals and infected in vitro. The inhibition of NETs production increased virus replication in neutrophils. In parallel, we observed that NETs inhibited virus abilities to infect and replicate in epithelial cells after 24 hours of infection. Degradation of NETs with DNase I prevented their virucidal effect in vitro. Using K18-humanized ACE2 transgenic mice, we observed a higher viral load in animals treated with DNase I. However, the virucidal effect of NETs was not dependent on neutrophil elastase or myeloperoxidase activity.
Conclusions: Our results provide evidence of the role of NETosis as a mechanism of SARS-CoV-2 viral capture and inhibition.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/infdis/jiad526 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CLINICAL PRESENTATION OF BONE METASTASES IN NEUROENDOCRINE TUMORS: A RETROSPECTIVE COHORT STUDY.
Endocr Relat Cancer
September 2025
Endocrinology, Diabetology and Medical Andrology Unit, IRCCS, Humanitas Research Hospital, Rozzano, Milan, Italy.
Bone metastases (BMs) are rare and late event in patients with neuroendocrine tumors (NETs). The aim of our study was to investigate clinical presentation and outcome of BMs in a large cohort of patients with NETs. A retrospective study was performed at two referral centers of Northern Italy (IRCCS Humanitas Research Hospital in Milan and S.
View Article and Find Full Text PDFPancreatic Involvement by Mixed Serous Neuroendocrine Neoplasm Detected by Endosonography With Fine-Needle Tissue Acquisition in Von Hippel-Lindau Disease.
Cureus
August 2025
Surgery and Anatomy, Hospital das Clinicas da Faculdade de Medicina de Ribeirão Preto - Universidade de São Paulo, Ribeirão Preto, BRA.
The Von Hippel-Lindau disease (VHL) is an autosomal dominant condition characterized by multiple cystic tumors in several organs, including the pancreas. The symptoms are variable, and suspicion must be raised with typical lesions, such as a hemangioblastoma of the central nervous system (CNS) or retina, associated with a renal cell carcinoma, a pheochromocytoma or multiple pancreatic cysts, besides neuroendocrine tumors (NET). The diagnosis in a patient without a family history should be suspected in case of a hemangioblastoma of the CNS and/or retina, which could also be associated with other lesions, such as pancreatic cysts and NETs.
View Article and Find Full Text PDFKaempferol as a multifaceted immunomodulator: implications for inflammation, autoimmunity, and cancer.
Front Immunol
September 2025
Department of Geriatrics, Jilin Geriatrics Clinical Research Center, The First Hospital of Jilin University, Changchun, Jilin, China.
Kaempferol (KMF) is a dietary flavonoid exhibiting profound immunomodulatory effects across multiple immune cell populations. This review synthesizes current insights into how KMF regulates diverse immune cell populations and its therapeutic potential in inflammatory and immune-related disorders. KMF exhibits multifaceted effects on T cells.
View Article and Find Full Text PDFAdaptive radiotherapy dose prediction on head and neck cancer patients with a 3D multi-headed U-Net deep learning architecture.
Mach Learn Health
December 2025
Medical Artificial Intelligence and Automation Laboratory, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, United States of America.
Online adaptive radiation therapy (ART) personalizes treatment plans by accounting for daily anatomical changes, requiring workflows distinct from conventional radiotherapy. Deep learning-based dose prediction models can enhance treatment planning efficiency by rapidly generating accuracy dose distributions, reducing manual trial-and-error and accelerating the overall workflow; however, most existing approaches overlook critical pre-treatment plan information-specifically, physician-defined clinical objectives tailored to individual patients. To address this limitation, we introduce the multi-headed U-Net (MHU-Net), a novel architecture that explicitly incorporates physician intent from pre-treatment plans to improve dose prediction accuracy in adaptive head and neck cancer treatments.
View Article and Find Full Text PDFEnriched environment requires the remodeling of hippocampal perineuronal nets to trigger memory improvement in Alzheimer's mouse model.
iScience
September 2025
University Toulouse, CNRS, Centre de Recherches sur la Cognition Animale, Centre de Biologie Intégrative, Toulouse, France.
Alzheimer's disease (AD) is a major neurodegenerative disorder influenced by both genetic and environmental factors. Mentally stimulating activities are believed to reduce cognitive decline by establishing a cognitive reserve, although the underlying neurobiological mechanisms remain elusive. In this study, we investigate the role of parvalbumin-expressing inhibitory neurons (PV+) and their perineuronal nets (PNN) in the establishment of cognitive reserve using a mouse model of AD.
View Article and Find Full Text PDF