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Article Abstract
Root nodule endosymbioses between diazotrophic rhizobia and legumes provide the largest input of combined N to the biosphere, thus representing an alternative to harmful chemical fertilizers for sustainable crop production. Rhizobia have evolved intricate strategies to coordinate N assimilation for their own benefit with N fixation to sustain plant growth. The rhizobial N status is transduced by the NtrBC two-component system, the seemingly ubiquitous form of N signal transduction in Proteobacteria. Here, we show that the regulatory sRNA NfeR1 (nodule formation efficiency RNA) of the alfalfa symbiont is transcribed from a complex promoter repressed by NtrC in a N-dependent manner and feedback silences by complementary base-pairing. These findings unveil a more prominent role of NtrC as a transcriptional repressor than hitherto anticipated and a novel RNA-based mechanism for NtrBC regulation. The NtrBC-NfeR1 double-negative feedback loop accurately rewires symbiotic N metabolism and is likely conserved in α-rhizobia.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746234 | PMC |
| http://dx.doi.org/10.1128/mbio.02003-23 | DOI Listing |
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