[Identification of Complex and Combined Antibody Consisted of Anti-c, Anti-E, Anti-Jk and Anti-Fy].

Objective: To investigate the role of multiple serological methods in the identification of complex antibodies.

Methods: The blood group antigens were detected by saline and microcolumn agglutination methods. The saline method was used to screen and identify IgM-type antibodies in the patient's serum, while the polybrene, anti-globulin, microcolumn agglutination, enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies.

Results: The patient was B/CCDee/Jk(a-b+)/Fy(a-b+) blood type. The serum reacted with panel cells, and the reaction presented anti-E pattern in the saline medium. It was fully positive in the microcolumn agglutination card, except 2 negative ones after using papain to treat the panel cells. Referring to the pattern table, it was concluded that there existed anti-c, anti-E, and anti-Jk antibodies, and one antibody corresponding to an antigen that was easily destroyed by papain. The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c, anti-E, anti-Jk and anti-Fy antibodies.

Conclusion: It is confirmed that IgM-type anti-E, and IgG-type anti-c, anti-E, anti-Jk and anti-Fy antibodies exist in the patient's serum by multiple serological methods.