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http://dx.doi.org/10.1016/j.slasd.2023.10.001 | DOI Listing |
Nanoscale Horiz
September 2025
Research Center of Nanomedicine Technology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Cuproptosis relies on intracellular copper accumulation and shows great potential in tumor therapy. However, the high content of glutathione (GSH) in tumor cells limits its effectiveness. Furthermore, the mechanism of immune activation mediated by cuproptosis remains unclear.
View Article and Find Full Text PDFFront Toxicol
August 2025
Ncardia Services B.V., Leiden, Netherlands.
Introduction: Efficient preclinical prediction of cardiovascular side effects poses a pivotal challenge for the pharmaceutical industry. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are becoming increasingly important in this field due to inaccessibility of human native cardiac tissue. Current preclinical hiPSC-CMs models focus on functional changes such as electrophysiological abnormalities, however other parameters, such as structural toxicity, remain less understood.
View Article and Find Full Text PDFMol Pharmacol
August 2025
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland. Electronic address:
Although multiparameter cellular morphological profiling methods and three-dimensional (3D) biological model systems can potentially provide complex insights for pharmaceutical discovery campaigns, there have been relatively few reports combining these experimental approaches. In this study, we used the U87 glioblastoma cell line grown in a 3D spheroid format to validate a multiparameter cellular morphological profiling screening method. The steps of this approach include 3D spheroid treatment, cell staining, fully automated digital image acquisition, image segmentation, numerical feature extraction, and multiple machine learning approaches for cellular profiling.
View Article and Find Full Text PDFArch Toxicol
September 2025
Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, H3G 1Y6, Canada.
Organophosphate esters (OPEs), commonly used as flame retardants and plasticizers, are ubiquitous environmental contaminants, with high concentrations found in indoor house dust. Previously, we have reported that individual OPEs have adverse effects on HepG2 liver cells. However, real-world exposure involves mixtures of OPEs.
View Article and Find Full Text PDFUnlabelled: Ovarian cancer is one of the most lethal gynecological cancers worldwide and has one of the highest recurrence rates. Recently developed Chimeric Antigen Receptor T (CAR-T) cell therapy has shown potent clinical efficacy against hematological malignancies. However, solid tumors, including ovarian cancer, possess several mechanisms that hinder T cell activity, and metabolic alteration of cancer cells has been shown to contribute to resistance to immune cell attack against solid tumors.
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