Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species. In this study, a recombinase polymerase amplification (RPA) assay was developed for the rapid and sensitive detection of the pathogens responsible for posing significant threats to the kiwifruit industries. The RPA primer pairs tested in this study were highly specific for detection of B. dothidea and D. eres. The detection limits of our RPA assays were approximately two picograms of fungal genomic DNA. The optimal conditions for the RPA assays were determined to be at a temperature of 39°C maintained for a minimum duration of 5 min. We were able to detect the pathogens from kiwifruit samples inoculated with a very small number of conidia. The RPA assays enabled specific, sensitive, and rapid detection of B. dothidea and D. eres, the primary pathogens responsible for kiwifruit rots in South Korea.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580058 | PMC |
| http://dx.doi.org/10.5423/PPJ.NT.07.2023.0094 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ecological effects of C3 in kiwifruit rhizosphere soil and its prevention and control against root rot disease.
Front Microbiol
August 2025
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan, China.
As the world's largest producer of kiwifruit, China faces significant yield and quality losses due to the widespread occurrence of kiwifruit root rot. To explore alternative biological control strategies for kiwifruit root rot, this study isolated 11 fungal isolates from diseased kiwifruit roots and identified as the primary pathogen. Additionally, a biocontrol strain, C3, was isolated from the rhizosphere of healthy kiwifruit and shown to significantly inhibit pathogen growth.
View Article and Find Full Text PDFOmics insights into LMY3-5 defense against in kiwifruit.
Front Microbiol
August 2025
Guizhou Botanical Garden, Guiyang, China.
is the main cause of soft rot in kiwifruit, significantly reducing both yield and quality. While chemical treatments are commonly used, their effectiveness is limited and they may pose environmental risks. As a result, biological control using Bacillus species has emerged as a promising alternative.
View Article and Find Full Text PDFFirst Report of Tomato Sour Rot Caused by in Heilongjiang Province, China.
Plant Dis
July 2025
Northeast Agricultural University, College of Agriculture, 600 changjiang road, Harbin, China, 150030;
Tomato sour rot, caused by Geotrichum candidum, is a postharvest disease. It has been detected across major tomato-producing regions in temperate to subtropical zones (e.g.
View Article and Find Full Text PDFPectinase-triggered metal-organic framework nanocarriers gated with pectin for postharvest kiwifruit preservation: Antifungal targeting and biosafety reinforcement.
Int J Biol Macromol
August 2025
Engineering & Technology Research Center for Bio Pesticide and Formulating Processing, College of Plant Protection, Hunan Agricultural University, Changsha 410000, China. Electronic address:
Smart pesticide delivery systems that respond to pathogen-specific enzymes represent a sustainable and targeted approach for managing postharvest diseases. In this study, we developed a pectinase-responsive nanoplatform (CYM@MOF@pec) by encapsulating cymophenol (CYM) within aminated metal-organic frameworks (UiO-66-NH₂), followed by surface coating with pectin as a bioactive gatekeeper. The resulting nanoparticles exhibited a uniform spherical morphology with an average diameter of 242 nm and a drug loading efficiency of 15.
View Article and Find Full Text PDFDesign and Synthesis of Ferulic Acid Derivatives with Trifluoromethyl Pyrimidine and Amide Frameworks for Combating Postharvest Kiwifruit Soft Rot Fungi.
J Agric Food Chem
June 2025
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Fungal diseases significantly affect the quality and safety of food and agricultural products. Pyrimidine ether compounds have shown effectiveness as commercial antifungal agents, but their prolonged or excessive use has led to resistance and pesticide residue issues, necessitating structural modifications. In this study, ferulic acid served as a framework for synthesizing a series of derivatives by introducing pyrimidine and amide groups.
View Article and Find Full Text PDF