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Microplastic pollution has become an environmental problem that cannot be ignored in our society. Raman spectroscopy technology has been widely used in the field of microplastics detection due to its non-contact, non-destructive chemical specificity. Traditional point confocal Raman micro-spectroscopy technology uses single-point detection, resulting in long measurement times to scan the large areas of interest of typical samples. In this paper, we present a line scan confocal Raman micro-spectroscopy tool for fast detection and identification of microplastic particles. We show size and composition identification of particles and imaging over large areas. Compared with point confocal Raman imaging, the line scan confocal Raman technology increases the imaging speed by 1-2 orders of magnitude.
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http://dx.doi.org/10.1016/j.talanta.2023.125067 | DOI Listing |
J Vis Exp
August 2025
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology.
We present multimodal confocal Raman micro-spectroscopy (RS) and tomographic phase microscopy (TPM) for quick morpho-chemical phenotyping of human breast cancer cells (MDA-MB-231). Leveraging the non-perturbative nature of these advanced microscopy techniques, we captured detailed morpho-molecular data from living, label-free cells in their native physiological environment. Human bias-free data processing pipelines were developed to analyze hyperspectral Raman images (spanning Raman modes from 600 cm to 1800 cm, which uniquely characterize a wide range of molecular bonds and subcellular structures), as well as morphological data from three-dimensional refractive index tomograms (providing measurements of cell volume, surface area, footprint, and sphericity at nanometer resolution, alongside dry mass and density).
View Article and Find Full Text PDFFood Chem
September 2025
College of Food Science and Technology, Whole Grain Food Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China; The Sanya Institute of Nanjing Agricultural University, Sanya 572024, People's Republic of China. Electronic address: wangpei@nj
Selectively hydrolyzed soy protein can enhance wheat-based product quality by modulating gluten thermal polymerization. This study examined the effects of β-conglycinin (7S) and glycinin hydrolysate (GH) on gluten rheological and thermal properties, particle size, Raman spectra, and microstructure during heating. Both 7S and GH improved gluten viscoelasticity, with their combined addition (7S/GH) showing the strongest effect.
View Article and Find Full Text PDFJ Control Release
September 2025
LEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark. Electronic address:
In situ forming implants (ISFIs) present a promising approach for sustained parenteral drug delivery, offering reduced first pass metabolism, fewer systemic side effects, and improved patient compliance. This study investigates defocused spatially-offset Raman spectroscopy (SORS) as a real-time, non-invasive, and label-free method for monitoring implant formation and drug release from ISFIs loaded with the model drugs 4-cyanophenol (4-CP) or all-trans retinoic acid (RA). A custom-designed flow-through diffusion cell, incorporating full-thickness porcine skin, was developed to enable ex vivo release studies, allowing simultaneous defocused SORS measurements of subcutaneously-implanted formulations and HPLC quantification of drug released into the receptor medium.
View Article and Find Full Text PDFSci Rep
August 2025
Center for Integrated Nanotechnologies, Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
While CdZnTe (CZT) and CdZnTeSe (CZTS) semiconductors have emerged as compounds for room-temperature gamma and X-ray detection materials, they continue to be constrained by the formation of Te-inclusion defects generated during the growth and post-growth phases of the material, which adversely affect the detector performance. We demonstrate the utility of multimodal microscopic imaging and analysis for the characterization of the optical and electronic properties of Te inclusions in CZT and CZTS crystals at both micron and nanometer length scales. Having first identified regions with micron-scale Te inclusions using confocal Raman microscopy techniques, optically coupled infrared scattering near-field optical microscopic mapping was performed to map the distribution of these inclusions with nanometer spatial resolution and correlate the presence of Te inclusions in the matrix with other properties.
View Article and Find Full Text PDFChem Biomed Imaging
August 2025
School of Materials Science and Engineering, Anhui University, 111 Jiulong Road, Hefei 230601, China.
Carbon dots (CDs) have emerged as promising nanomaterials for bioimaging and stress monitoring due to their unique optical and functional properties. CDs were synthesized using citric acid and -phenylenediamine via microwave-assisted heating, named as CP-CDs. High-resolution transmission electron microscopy observed an average particle size of 3.
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