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http://dx.doi.org/10.1093/plphys/kiad436 | DOI Listing |
J Org Chem
September 2025
Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, P. R. of China.
A Mg(OTf)-catalyzed asymmetric Michael addition/cyclization cascade reaction between 3-isothiocyanato oxindoles and 2-arylidene-1,3-indanediones has been developed. This transformation provides an efficient and concise approach to biologically important bispiro[indanedione-oxindole-pyrrolidinyl]s under mild conditions in good to excellent yields (70-99% yields) with moderate to good stereoselectivities (up to 99% and >95:5 d.r.
View Article and Find Full Text PDFJ Proteome Res
September 2025
School of Basic Medical Sciences, Institute of Biomedical Innovation, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi Province 330031, China.
Extracellular vesicles (EVs) are membranous structures consisting of lipid bilayers that are released by most cell types and serve as important mediators of intercellular communication. The HEK293T cell line model has gained considerable attention from the scientific community, particularly in the fields of engineering and drug delivery. Nevertheless, there is a dearth of systematic comparisons of the most prevalent EV isolation methodologies for HEK293T in terms of recovery and specificity.
View Article and Find Full Text PDFJ Appl Microbiol
September 2025
Sivas Cumhuriyet University, Faculty of Medicine, Department of Medical Microbiology, 58140 Sivas, Türkiye.
Aims: The increasing antimicrobial resistance, particularly in Acinetobacter baumannii, complicates the treatment of infections, leading to higher morbidity, mortality, and economic costs. Herein, we aimed to determine the in vitro antimicrobial, synergistic, and antibiofilm activities of colistin (COL), meropenem, and ciprofloxacin antibiotics, and curcumin, punicalagin, geraniol (GER), and linalool (LIN) plant-active ingredients alone and in combination against 31 multidrug-resistant (MDR) A. baumannii clinical isolates.
View Article and Find Full Text PDFPhysiol Plant
September 2025
Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, Saskatchewan, Canada.
Dormancy release and germination of the seed are two separate, but continuous phases controlled by both external (e.g., light and temperature) and internal (e.
View Article and Find Full Text PDFPhysiol Plant
September 2025
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, School of Sciences of the University of Minho, Braga, Portugal.
The Mediterranean Basin, a hotspot for tomato production, is one of the most vulnerable areas to climate change, where rising temperatures and increasing soil and water salinization represent major threats to agricultural sustainability. Thus, to understand the molecular mechanisms behind plant responses to this stress combination, an RNA-Seq analysis was conducted on roots and shoots of tomato plants exposed to salt (100 mM NaCl) and/or heat (42°C, 4 h each day) stress for 21 days. The analysis identified over 8000 differentially expressed genes (DEGs) under combined stress conditions, with 1716 DEGs in roots and 2665 in shoots being exclusively modulated in response to this specific stress condition.
View Article and Find Full Text PDF