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This study examined continuous mixed-culture microalgae cultivation for nutrient removal from anaerobic digestion (AD) effluents in photobioreactors, while altering the NH-N loading rate (NLR) by adjusting either the hydraulic retention time (HRT) (reactor set RH) or the influent NH-N concentration (reactor set RS). Both RH and RS demonstrated efficient nutrient removal and microalgae cultivation at NLRs of 4-10 mg NH-N/L∙d, reaching peak performance at 10 mg NH-N/L∙d. Within this range, RH obtained greater biomass yield and productivity, while RS maintained higher microalgal concentrations. The cultivated biomasses obtained from RH and RS had good settleability and suitable fatty acid compositions as a biodiesel feedstock, although their organic composition varied considerably with NLR and HRT. Parachlorella overwhelmingly dominated the reactors' microalgal communities throughout the experiment, co-existing with various microalgae-associated bacteria. Changes in NLR significantly influenced the bacterial community structures, underscoring its critical role in determining reactor performance and microalgal-bacterial community behavior.
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http://dx.doi.org/10.1016/j.biortech.2023.129650 | DOI Listing |
Environ Technol
August 2025
Universidad Católica San Pablo de Arequipa, Arequipa, Peru.
This study evaluated the suitability of wastewater treatment from tanneries located in the city of Arequipa, Peru. The growth of the microalgae-bacteria consortium enriched from wastewater ponds located in high altitude (2300 m above sea level) and areas with high ultraviolet radiation, was characterized under different wastewater dilutions, nutrient-corrected wastewater and various light periods in a laboratory-scale air-lift system over a 9-day cultivation period . The results indicate a higher COD removal at low dilutions (50%) in both 16L:8D and 24L:0D photoperiods, achieving 43.
View Article and Find Full Text PDFJ Environ Manage
March 2025
CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. Electronic address:
External aeration significantly influences microalgae consortium performance in municipal wastewater treatment. This study evaluated two Chlorella strains and mixed cultures under daytime, nighttime, and continuous aeration modes at 50 and 200 mL/min. Distinct aeration preferences were observed among microalgal strains, necessitating tailored strategies for mixed microalgae.
View Article and Find Full Text PDFInt J Phytoremediation
June 2025
Department of Ecology and Environmental Science, Assam University, Silchar, Assam, India.
Chemosphere
July 2024
Instituto de Energías Renovables, Universidad Nacional Autónoma de México (IER-UNAM), Priv. Xochicalco S/N, Col. Centro, Temixco, Morelos, 62580, Mexico. Electronic address:
The present study demonstrates, for the first time, the feasibility of a two-step process consisting of Electro-Fenton (EF) followed by microalgae to treat highly loaded real food processing wastewater along with resource recovery. In the first step, EF with a carbon felt cathode and Ti/RuO-IrO anode was applied at different current densities (3.16 mA cm, 4.
View Article and Find Full Text PDFWater Res
June 2024
Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ 85287-5701, United States of America.
Extracellular polymeric substances (EPS) play significant roles in the formation, function, and interactions of microalgal-bacteria consortia. Understanding the key roles of EPS depends on reliable extraction and quantification methods, but differentiating of EPS from microalgae versus bacteria is challenging. In this work, cation exchange resin (CER) and thermal treatments were applied for total EPS extraction from microalgal-bacteria mixed culture (MBMC), flow cytometry combined with SYTOX Green staining was applied to evaluate cell disruption during EPS extraction, and auto-fluorescence-based cell sorting (AFCS) was used to separate microalgae and bacteria in the MBMC.
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