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In this issue of , Sunden et al. develop an enzymatic assay to measure UDP-GlcNAc levels from cells and tissue. By reporting on the level of the substrate itself, this approach can potentially enhance the fields' understanding of UDP-GlcNAc concentration under a variety of conditions.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391555 | PMC |
http://dx.doi.org/10.1016/j.crmeth.2023.100537 | DOI Listing |
Angew Chem Int Ed Engl
September 2025
Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610065, China.
Detection methods with single-nucleotide specificity are essential tools for nucleic acid analysis in diverse clinical and biological settings. However, both hybridization-based and enzyme-based methods are only effective for discriminating single-nucleotide mutations at certain positions, making it difficult to detect nucleic acid targets having multiple nearby mutations. Herein, we describe the design of cooperative recognition probes (CRPs) that integrate both hybridization and ligation-based recognition mechanisms and thus are highly effective for discriminating mutations throughout all positions.
View Article and Find Full Text PDFRSC Adv
August 2025
Instituto de Ciencias, Universidad Nacional de General Sarmiento - CONICET Los Polvorines Argentina
We present a biomimetic electrochemical sensor for glyphosate (GLY) detection, utilizing graphite electrodes modified with electropolymerized copper(ii) meso-tetra(4-sulfonatophenyl)porphyrin (CuP). The Cu(ii) centers provide dual functionality: catalytic oxygen reduction and selective GLY coordination, which leads to a proportional suppression of redox currents. Characterization (SEM-EDS/Raman/UV-Vis) confirmed CuP polymerization and specific GLY binding.
View Article and Find Full Text PDFBiosensors (Basel)
July 2025
Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, Japan.
Histamine sensing that uses enzymatic reactions is the most common form of testing due to its selectivity for histamine. However, enzymes are difficult to store for long periods of time, and the inactivation of enzymes decreases the reliability of the results. In this study, we developed a novel, quick, and easily operated histamine sensing technique that takes advantage of the histamine redox reaction and does not require enzyme-based processes.
View Article and Find Full Text PDFACS Meas Sci Au
August 2025
São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos 13560-970, Brazil.
The development of enzyme-based bioelectronic devices, including biosensors and biomimetic systems, has significantly advanced with the introduction of innovative materials such as hydrogels, deep eutectic solvents (DES), and ionic liquids (ILs). These materials offer unique advantages in enhancing biodevice performance, particularly in enzyme stabilization, biocompatibility, and electrochemical sensitivity. Hydrogels, known for their high water content and flexibility, provide an ideal matrix for enzyme immobilization in biological applications but are limited by low ionic conductivity.
View Article and Find Full Text PDFTalanta
August 2025
School of Automation and Intelligent Sensing, Shanghai Jiao Tong University, Shanghai, 200240, China; School of Life Science and Biotechnology, Key State Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:
The concept of visual enzyme inhibition-electrophoresis titration (EI-ET) was proposed for design of moving reaction boundary based portable EI-ET chip device without use of traditional optical detector and power supply. Theoretical results revealed that a series of enzyme inhibition and kinetic parameters could be obtained via an EI-ET run. As a proof of concept, horseradish peroxidase and Cu(I) were respectively chosen as model enzyme and inhibitor for the experiments of EI-ET.
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