Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

The introduction of internet-connected technologies to the classroom has the potential to revolutionize STEM education by allowing students to perform experiments in complex models that are unattainable in traditional teaching laboratories. By connecting laboratory equipment to the cloud, we introduce students to experimentation in pluripotent stem cell-derived cortical organoids in two different settings: Using microscopy to monitor organoid growth in an introductory tissue culture course, and using high density multielectrode arrays to perform neuronal stimulation and recording in an advanced neuroscience mathematics course. We demonstrate that this approach develops interest in stem cell and neuroscience in the students of both courses. All together, we propose cloud technologies as an effective and scalable approach for complex project-based university training.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369936PMC
http://dx.doi.org/10.1101/2023.07.13.546418DOI Listing

Publication Analysis

Top Keywords

cortical organoids
8
stem cell
8
cell neuroscience
8
internet-connected cortical
4
organoids project-based
4
stem
4
project-based stem
4
neuroscience education
4
education introduction
4
introduction internet-connected
4

Similar Publications

Cortical versus hippocampal network dysfunction in a human brain assembloid model of epilepsy and intellectual disability.

Cell Rep

September 2025

Department of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA; Department of Neurobiology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Un

Neurodevelopmental disorders often impair multiple cognitive domains. For instance, a genetic epilepsy syndrome might cause seizures due to cortical hyperexcitability and present with memory impairments arising from hippocampal dysfunction. This study examines how a single disorder differentially affects distinct brain regions using induced pluripotent stem cell (iPSC)-derived cortical- and hippocampal-ganglionic eminence assembloids to model developmental and epileptic encephalopathy 13, a condition arising from gain-of-function mutations in the SCN8A gene encoding the sodium channel Nav1.

View Article and Find Full Text PDF

Recent advances in three-dimensional (3D) biological brain models in vitro and ex vivo are creating new opportunities to understand the complexity of neural networks but pose the technological challenge of obtaining high-throughput recordings of electrical activity from multiple sites in 3D at high spatiotemporal resolution. This cannot be achieved using planar multi-electrode arrays (MEAs), which contact just one side of the neural structure. Moreover, the specimen adhesion to planar MEAs limits fluid perfusion along with tissue viability and drug application.

View Article and Find Full Text PDF

Maternal low thyroxine (T4) serum levels during the first trimester of pregnancy correlate with cerebral cortex volume and mental development of the progeny, but why neural cells during early fetal brain development are vulnerable to maternal T4 levels remains unknown. In this study, using iPSCs obtained from a boy with a loss-of-function mutation in MCT8-a transporter previously identified as critical for thyroid hormone uptake and action in neural cells-we demonstrate that thyroid hormones induce transcriptional changes that promote the progression of human neural precursor cells along the dorsal projection trajectory. Consistent with these findings, single-cell, spatial, and bulk transcriptomics from MCT8-deficient cerebral organoids and cultures of human neural precursor cells underscore the necessity for optimal thyroid hormone levels for these cells to differentiate into neurons.

View Article and Find Full Text PDF

Traumatic brain injury (TBI) is the most important environmental risk factor for neurodegenerative disease. Tauopathy plays an important role in post-traumatic neurodegeneration. Human-induced pluripotent stem cell (hiPSC)-derived cortical organoids have exciting potential to reveal the influence of genotype on post-traumatic neurodegeneration because they permit manipulation of the genome in a human system.

View Article and Find Full Text PDF

Introduction: Human iPSC-derived brain organoids and assembloids have emerged as promising in vitro models for recapitulating human brain development, neurological disorders, and drug responses. However, detailed analysis of their electrophysiological properties requires advanced measurement techniques.

Methods: Here, we present an analytical approach using ultra-high-density (UHD) CMOS microelectrode arrays (MEAs) with 236,880 electrodes across a 32.

View Article and Find Full Text PDF