Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial- mesenchymal transition (EMT) in ovarian adenocarcinoma cells.
Methods: The ovarian adenocarcinoma cell line SKOV3 was used as a TGFβ1-induced EMT model. Cells were incubated with 5 ng/mL TGFβ1 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers. Western blot was used to analyze phospho- and total MAPK protein levels. Typical and atypical DUSPs mRNA expression profile was determined by real-time qPCR.
Results: The epithelial marker E-cadherin expressions were decreased and mesenchymal EMT markers Snail and Slug expression levelswere increased after TGFβ1 induction. Phosphorylation of ERK1/2 and p38 MAPK were enhanced in response to TGFβ1 treatment. The expression of DUSP2, DUSP6, DUSP8, DUSP10, and DUSP13 were decreased while DUSP7, DUSP16, DUSP18, DUSP21, and DUSP27 were increased by TGFβ1.
Discussion: TGFβ1 induced EMT which was accompanied by increased activity of MAPKs, and led to marked changes in expressions of several DUSPs in SKOV3 cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387886 | PMC |
| http://dx.doi.org/10.55730/1300-0144.5626 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Manipulation of macrophage signaling by virulence factors.
Virulence
December 2025
Clinical HIV Laboratory, JSPS Government Homeopathic Medical College, Hyderabad, Telangana, India.
, a macrophage-residing parasite, expresses virulence factors that intercept macrophage signaling and inflicts leishmaniasis. Recently described virulence factors- eEF-1α (eukaryotic elongation factor), LmjF_36_3850 ( F_36_3850), LdTyrPIP_22 (LDBPK_220120.1) and LmjMAPK ( mitogen activated protein kinase)-4/12 selectively modulate the activities of kinases, phosphatases and metabolism of phosphatidylinositol influencing the infection outcome.
View Article and Find Full Text PDFIntrinsically disordered regions in atypical dual-specificity phosphatases: A review.
Int J Biol Macromol
August 2025
Laboratory of Signaling in Biomolecular Systems, Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Brazil. Electronic address:
Intrinsically disordered regions (IDRs) are conserved elements that enable cellular adaptability by mediating rapid responses to environmental cues. Their biophysical properties-disorder-to-order transitions, phase separation, and interaction versatility-are vital for protein localization, signaling, and disease mechanisms. In protein tyrosine phosphatases (PTPs), and notably dual-specificity phosphatases (DUSPs), IDRs serve functional roles beyond passive linkers.
View Article and Find Full Text PDFCell-specific expression and signaling of glucocorticoid receptor isoforms over time in critically ill patients with a low inflammatory response.
Crit Care
August 2025
1st Department of Critical Care Medicine, Evangelismos Hospital, School of Medicine, National & Kapodistrian University of Athens, Athens, Greece.
Background: Glucocorticoid (GC) signaling plays a crucial role in immune regulation during critical illness, but cell-specific responses remain poorly understood. While previous studies have predominantly examined glucocorticoid receptor (GCR)-α and GCR-β, the roles of alternative isoforms (GCR-γ, GCR-P) and the downstream effectors GC-induced leucine zipper (GILZ) and dual-specific phosphatase 1 (DUSP1) across different immune cell populations in critical illness remain unexplored.
Methods: In this prospective, observational study, we enrolled 43 critically ill patients and 25 healthy controls.
IER5 Negatively Regulates Cdc25B Expression in HeLa Cells After Gamma Ray Irradiation.
Biomed Res Int
August 2025
National Institute for Radiological Protection, China CDC, Beijing, China.
Radiotherapy is commonly used to treat many cancers, and their sensitivity to radiation is crucial for favorable outcomes. This study investigated whether the immediate early response 5 (IER5) protein affects Cdc25B protein expression in HeLa cells after gamma irradiation. IER5 was knocked down using RNA interference (RNAi) in HeLa cells irradiated with 2 or 4 Gy of gamma rays.
View Article and Find Full Text PDF[Interferon-λ1 improves glucocorticoid resistance caused by respiratory syncytial virus by regulating the p38 mitogen-activated protein kinase signaling pathway].
Zhongguo Dang Dai Er Ke Za Zhi
August 2025
Children's Medical Center, Hunan Provincial People's Hospital/First Affiliated Hospital of Hunan Normal University/Hunan Key Laboratory of Pediatric Respiratory Diseases, Changsha 410005, China.
Objectives: To investigate the effect of interferon-λ1 (IFN-λ1) on glucocorticoid (GC) resistance in human bronchial epithelial cells (HBECs) stimulated by respiratory syncytial virus (RSV).
Methods: HBECs were divided into five groups: control, dexamethasone, IFN-λ1, RSV, and RSV+IFN-λ1. CCK-8 assay was used to measure the effect of different concentrations of IFN-λ1 on the viability of HBECs, and the sensitivity of HBECs to dexamethasone was measured in each group.