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Bacterial vaginosis (BV) is the most common vaginal infection worldwide. We developed a peptide nucleic acid (PNA) probe targeting Prevotella bivia, a common BV-associated bacteria, and optimized a multiplex approach for detection of Gardnerella spp., P. bivia and Fannyhessea vaginae. Our P. bivia PNA probe specifically detected the target species, and the optimized multiplex approach was able to detect the presence of the three species in multi-species BV biofilms.
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http://dx.doi.org/10.1038/s41522-023-00411-6 | DOI Listing |
Anal Biochem
September 2025
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-Cho Minami, Tottori, 680-8552, Japan.
The duplex-forming behavior of an inchworm-type PNA-PEG conjugate (i-PPc), engineered for the selective recognition of point mutations in DNA, was assessed through thermodynamic analysis employing UV melting curves and circular dichroism spectroscopy. The i-PPc demonstrated the ability to form stable duplexes exclusively with fully complementary DNA sequences, while no hybridization with single-base mismatched sequences. This binary on/off hybridization behavior was maintained even under physiologically relevant conditions (37 °C), thereby illustrating the exceptional point mutation discrimination capability of i-PPc.
View Article and Find Full Text PDFACS Nano
August 2025
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, California 90089, United States.
The expression of distinct microRNA (miRNA) species is associated with many major diseases, and thus, stable and reliable detection at low concentrations is crucial for early diagnosis and treatment. Field-effect transistor (FET)-based biosensors have shown significant progress in miRNA detection, but detection at ultralow concentrations remains challenging due to weak signal generation from small, low-charge miRNA molecules. Here, we report an InO nanoribbon-based FET biosensor platform capable of detecting miRNAs at attomolar (aM) concentrations.
View Article and Find Full Text PDFMutat Res Genet Toxicol Environ Mutagen
July 2025
Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular (IMBICE, CONICET-UNLP-CICPBA), calle 526 y Camino General Belgrano, La Plata, Buenos Aires B1906APO, Argentina; Universidad Nacional de La Plata, Facultad de Ciencias Naturales y Museo, calle 60 y 122, La
The induction of telomere dysfunction-related chromosomal aberrations by the radiomimetic antibiotic bleomycin (BLM) was studied in human lymphoblastoid cells immortalized with the Epstein-Barr virus (EBV). To this end, an EBV-induced lymphoblastoid cell line (T-37) was exposed to increased concentrations of BLM (10-100 µg/mL) for 2 h at 37ºC, and telomere aberrations were analyzed 24 h (first mitosis) after treatment using PNA-FISH with pan-telomeric plus pan-centromeric probes. Telomere signal duplications (TSD) increased significantly in BLM-exposed cells (p < 0.
View Article and Find Full Text PDFMethods
October 2025
Department of Respiratory and Critical Care Medicine, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou 215028, China. Electronic address:
In this work, an electrochemiluminescence (ECL) biosensor integrating T7 RNA polymerase amplification and CRISPR/Cas13a-mediated signal enhancement was developed for the ultrasensitive detection of matrix metalloproteinase-2 (MMP-2), a key biomarker associated with chronic inflammatory diseases such as COPD. A peptide nucleic acid (PNA) probe was designed to respond specifically to MMP-2 cleavage, enabling the release of DNA templates for subsequent T7 RNA polymerase-driven transcription amplification. The generated RNA triggers the collateral cleavage activity of CRISPR/Cas13a, resulting in a significant amplification of the ECL signal.
View Article and Find Full Text PDFMethods Mol Biol
July 2025
The Institute for Drug Research, The School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
Peptide nucleic acids (PNA) [1] are synthetic DNA analogs that are used as DNA/RNA mimics for diagnostic [2, 3] and therapeutic purposes [2-5]. One sensing strategy is the use of fluorogenic probes. One such probe is forced intercalation (FIT) PNA, which has a surrogate base as a cyanine dye (e.
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