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Article Abstract
Herein, we report an ATP-responsive nanoparticle ( NP) whose surface is fully covered with the biomolecular machine "chaperonin protein GroEL". NP was synthesized by DNA hybridization between a gold NP with DNA strands on its surface and GroEL carrying complementary DNA strands at its apical domains. The unique structure of NP was visualized by transmission electron microscopy including under cryogenic conditions. The immobilized GroEL units retain their machine-like function and enable NP to capture denatured green fluorescent protein and release it in response to ATP. Interestingly, the ATPase activity of NP per GroEL was 4.8 and 4.0 times greater than those of precursor GroEL and its DNA-functionalized analogue, respectively. Finally, we confirmed that NP could be iteratively extended to double-layered NP.
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