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, the generic type of belonging to , is a precious medicinal wood-inhabiting macrofungus with high commercial potential. To facilitate the medicinal utilization of this fungal resource, transcriptome sequences are newly generated from . strain MS2. In association with the previously generated genome sequences from the same strain by our lab and all available fungal homologous protein sequences in the UniProtKB/Swiss-Prot Protein Sequence Database, a new methodology was employed for genome assembly and annotation. A total of 13,531 protein-coding genes were identified from the new version of the genome of . strain MS2 with a complete BUSCOs of 92.8%, which indicates a remarkable improvement in the accuracy and completeness of the genome assembly. In general, more genes involved in medicinal functions were annotated compared with the original version of the genome annotation, and most of these genes were also found in the transcriptome data of the currently sampled growth period. Given the above, the current genomic and transcriptomic data provides valuable insights into the evolution and metabolites analysis of . .
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http://dx.doi.org/10.3390/jof9050505 | DOI Listing |
Medicina (Kaunas)
August 2025
Cardiac Care Unit, Intensive Care Unit, Jaén University Hospital, 23007 Jaén, Spain.
: The association between right ventricular myocardial fiber deformation and nutrition in weightlifters has not been fully characterized. This study analyzed nutritional factors and right ventricle speckle tracking echocardiography parameters in weightlifters before and after bench press exercises. : This interventional study examined the effects of bench press exercises on myocardial function.
View Article and Find Full Text PDFAnal Chem
September 2025
Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California 95064, United States.
Mass spectrometry imaging (MSI) is a powerful tool for monitoring the spatial distributions of microbial metabolites directly from culture. MSI can identify secretion and retention patterns for microbial metabolites, allowing for the assessment of chemical communication within complex microbial communities. Microbial imaging via matrix-assisted laser desorption/ionization (MALDI) MSI remains challenging due to high sample complexity and heterogeneity associated with the required sample preparation, making annotation of molecules by MS alone challenging.
View Article and Find Full Text PDFJ Proteome Res
September 2025
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, United States.
The effective separation of complex peptide mixtures is a cornerstone of mass spectrometry-based proteomics analysis as it enhances the accuracy and depth of proteomic analyses. Here, we compare data sets collected of whole-cell tryptic peptides, which were fractionated by either conventional flame-pulled, C18 packed-bed microcapillary columns or a microfabricated pillar array column (μPAC). Sixteen samples that included four different yeast strains (Δ, Δ, Δ, and wildtype) were analyzed in quadruplicate using data-independent acquisition.
View Article and Find Full Text PDFPLoS Genet
August 2025
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
Clostridioides difficile is the major cause of nosocomial infections associated with antibiotic therapy. The severity of C. difficile infections increased worldwide with the emergence of hypervirulent strains, including 027 ribotype epidemic strains.
View Article and Find Full Text PDFSci Rep
August 2025
Beijing Institute of Space Mechanics and Electricity, Beijing, 100094, China.
The traditional lunar landing buffering structure uses multi-stage aluminum honeycomb materials to absorb impact energy. However, due to irreversible deformation and abrupt acceleration changes during the buffering process, it fails to meet reusability and compliant buffering requirements. Additionally, star table detectors have short landing times, typically completing buffering and energy absorption within 1 s.
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