Molecular Epidemiological Investigation of spp. in Holstein Cattle in Partial Areas of the Yunnan Province, China.

spp. is a food-borne intestinal protozoan, which is widely distributed in the world and poses the risk of zoonosis. In order to reveal the prevalence of spp. in Holstein cattle in partial areas of the Yunnan Province, 524 fresh fecal samples of Holstein cattle were collected from Dali, Kunming, Chuxiong, and Qujing in Yunnan Province. A nested PCR amplification of the small subunit (SSU) rRNA gene of spp. was carried out, and the products of the nested PCR were further analyzed by restriction fragment length polymorphism (RFLP) using Ⅰ. The results of the present study showed that 13 samples were positive for spp., and the total infection rate of sp. was 2.48%. The infection of spp. was detected in Dali, Qujing, and Chuxiong. Chuxiong showed the highest infection rate (5.71%), and infection rate in Dali and Qujing was 2.19% and 3.16%, respectively. Interestingly, the infection of spp. was not detected in Kunming. The infection of spp. showed no significant differences among different regions ( > 0.05). sp. infection was detected in all ages and sexes, but the differences were not significant ( > 0.05). Sequencing and phylogenetic analysis showed that five spp. samples were closely related to the spp. of humans, and the others were closely related to the spp. of bovines. The results of the present study suggested that there was an infection of spp. in Holstein cattle in the Yunnan Province, and the spp. showed a risk of zoonosis. Thus, the prevention and control of spp. should be strengthened in the Yunnan Province, China. The results of this investigation provide data references for the further research of Cyclosporiasis in Holstein cattle in the Yunnan Province.