Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope is demonstrated. DNA Points Accumulation for Imaging in Nanoscale Topography with fluorogenic probes provides a suitable combination of background reduction and binding kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/smtd.202201565 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A ratiometric dual-channel fluorescent probe for selective Zn/Cd sensing: Applications in food quality control, real-time monitoring in living cells, and mice.
Anal Chim Acta
November 2025
State Key Laboratory of Materials-oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, PR China; Zhangjiagang Institute of Nanjing Tech University, Suzhou, 215600, PR China. Electronic address:
Background: Zinc (Zn) and cadmium (Cd) ions are ubiquitous in industrial and daily life. Despite their critical impact on food safety and human health, current probes face significant limitations in simultaneously detecting both ions in complex food matrices.
Results: Herein, we successfully developed a pyrene-based FRET ratiometric fluorescent probe QP for the highly selective detection of Zn and Cd.
A FRET ratiometric fluorescent probe for detection of bisulfite in food: Insights into food quality and preservation.
Anal Chim Acta
November 2025
School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China. Electronic address:
Background: Bisulfite (HSO) plays crucial roles in food safety and physiological health. In the food industry, sulfur dioxide (SO) and its derivative bisulfite (HSO) are extensively employed as preservatives and bleaching agents. Nonetheless, overconsumption of bisulfite can present health hazards like asthma and potentially cancer.
View Article and Find Full Text PDFA dansyl-quinolinium-based ratiometric fluorescent probe for detecting HSO/SO in living cells.
Anal Chim Acta
November 2025
Department of Obstetrics, The Second Hospital of Shandong University, Jinan, 250033, PR China. Electronic address:
Background: Sulfur dioxide (SO) is recognized as a major atmospheric pollutant and its excessive emissions can pose a great threat to the environment, flora and fauna, and human health. Long-term exposure to excessive SO can cause chronic poisoning, leading to neurological disorders and cardiovascular diseases. However, there are two sides to everything.
View Article and Find Full Text PDFIntegrating FRET and Molecular Dynamics Simulation for Single-Molecule Aptameric Detection of Staphylococcus aureus IsdA Surface Protein.
Biotechnol J
September 2025
Department of Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Staphylococcus aureus is ranked among the top five most common foodborne pathogens affecting public health and the economy worldwide. To improve detection and reduce diagnostic burdens, several detection methods from traditional culture-based techniques to biosensing platforms have evolved. Among several markers, surface proteins are considered to be the most important markers due to the specific roles they play in the survival and colonization of the bacterium on hosts.
View Article and Find Full Text PDFAutoFRET: An Image Processing-Based ROI Automated Selection Method for Quantitative FRET Measurements.
Microsc Microanal
September 2025
College of Photonics, School of Optoelectronic Science and Engineering, South China Normal University, Tianhe District, Guangzhou 510898, China.
The emission-based fluorescence resonance energy transfer (E-FRET), renowned for its rapid detection, noninvasiveness towards fluorophores, and compatibility with both wide-field and confocal microscopy, is extensively employed in dynamically monitoring intermolecular interactions within living cells. However, E-FRET requires manual screening of hundreds to thousands of images for regions meeting specific criteria, a labor-intensive process devoid of mature automation solutions. In this article, we introduce AutoFRET, the automated and efficient solution tailored for E-FRET experimentation.
View Article and Find Full Text PDF